1887

Abstract

A novel aerobic, Gram-negative bacterium, designated 3X/A02/235, was isolated from the surface of coastal waters in the north-western Mediterranean Sea. Cells were motile, straight rods, 1.6 μm long and 0.5 μm wide, and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 61 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the class and within the family . On the basis of 16S rRNA gene sequence comparisons and physiological and biochemical characteristics, this isolate represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 3X/A02/235 (=DSM 19537 =CIP 109602 =MOLA 286).

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2008-05-01
2020-10-01
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References

  1. Agogué, H., Casamayor, E. O., Joux, F., Obernosterer, I., Dupuy, C., Lantoine, F., Catala, P., Weinbauer, M. G., Reinthaler, T. & other authors ( 2004; ). Comparison of samplers for the biological characterization of the sea surface microlayer. Limnol Oceanogr Methods 2, 213–225.[CrossRef]
    [Google Scholar]
  2. Agogué, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., Herndl, G. J. & Lebaron, P. ( 2005; ). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54, 269–280.[CrossRef]
    [Google Scholar]
  3. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. ( 1997; ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef]
    [Google Scholar]
  4. Humm, H. J. ( 1946; ). Marine agar-digesting bacteria of the South Atlantic coast. Bull Duke Univ Mar Stn 3, 45–75.
    [Google Scholar]
  5. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  6. Palleroni, N. J. ( 1984; ). Genus Pseudomonas Migula 1894. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Powers, E. M. ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758.
    [Google Scholar]
  8. Raguénès, G., Christen, R., Guezennec, J., Pignet, P. & Barbier, G. ( 1997; ). Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef]
    [Google Scholar]
  9. Tang, Y. W., Ellis, N. M., Hopkins, M. K., Smith, D. H., Dodge, D. E. & Persing, D. H. ( 1998; ). Comparison of phenotypic and genotypic techniques for identification of unusual aerobic pathogenic gram-negative bacilli. J Clin Microbiol 36, 3674–3679.
    [Google Scholar]
  10. Urios, L., Agogué, H., Lesongeur, F., Stackebrandt, E. & Lebaron, P. ( 2006; ). Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. Int J Syst Evol Microbiol 56, 1883–1887.[CrossRef]
    [Google Scholar]
  11. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M.-A., Godfroy, A. & Barbier, G. ( 2001a; ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504.
    [Google Scholar]
  12. Wery, N., Moricet, J.-M., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M.-A. & Barbier, G. ( 2001b; ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef]
    [Google Scholar]
  13. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003a; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
  14. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H. ( 2003b; ). Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int J Syst Evol Microbiol 53, 1357–1361.[CrossRef]
    [Google Scholar]
  15. Yoon, J. H., Kim, I. G., Oh, T. K. & Park, Y. H. ( 2004; ). Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54, 1111–1116.[CrossRef]
    [Google Scholar]
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Electron micrograph of a negatively stained cell of strain 3X/A02/235 harvested during the exponential phase. A single polar flagellum is shown. Bar, 0.5 µm.

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Effects of temperature, pH and NaCl concentration on growth of strain 3X/A02/235 . [PDF](16 KB)

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