A bacterial strain designated GSPC1 was isolated from root nodules of in Segovia (Spain). The 16S rRNA gene sequence of this strain showed 95.9 and 94.7 % sequence similarity, respectively, with those of the type strains of and . Strain GSPC1 presented phenotypic, chemotaxonomic and molecular differences with respect to species which indicated that it belonged to a different species. The isolate was a Gram-positive, aerobic, sporulated rod, motile by means of peritrichous flagella. The strain was catalase-positive and showed weak oxidase activity. It grew in the presence of 2 % NaCl. MK-7 was the predominant menaquinone. anteiso-C, iso-C, iso-C and C were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content was 60.3 mol%. The results of this study suggest that isolate GSPC1 should be classified within a novel species, for which the name sp. nov. is proposed, with strain GSPC1 (=LMG 24086 =DSM 19269) as the type strain.


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Electron micrographs of strain GSPC1 grown in nutrient agar for 48 h (a), showing flagella, and 72 h (b), showing round spores. Bars, 2.5 µm.


Electron micrographs of strain GSPC1 grown in nutrient agar for 48 h (a), showing flagella, and 72 h (b), showing round spores. Bars, 2.5 µm.


Two-dimensional thin-layer chromatogram of polar lipids of strain GSPC1 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL1–PL4, unknown phospholipids; GL1 and GL2, unknown glycolipids; PN, unknown aminophosphoglycolipid.


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