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Volume 58, Issue 7

sp. nov., a moderate halophile from saline soil in China Free

Abstract

A moderately halophilic, Gram-positive coccus, designated strain W24, was isolated from saline soil in Qinghai province, China. The isolate was able to grow at salinities of 0–24 % (w/v) NaCl (optimally at 8 %, w/v), at pH 5.5–9.0 (optimally at pH 7.5) and at 8–43 °C (optimally at 28 °C). The genomic DNA G+C content of strain W24 was 45.8 mol%. The predominant isoprenoid quinone was MK-6 and the cell wall contained lysine and glycine as diagnostic diamino acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were iso-C, anteiso-C and C. Based on 16S rRNA gene sequence analysis, strain W24 was found to be a member of the genus and was related most closely to DSM 5352 (96.5 % sequence similarity). Based on data from the current polyphasic study, strain W24 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W24 (=CGMCC 1.6501=DSM 19336).

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2008-07-01
2024-04-23
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References

  1. Aslam, Z., Lim, J. H., Im, W. T., Yasir, M., Chung, Y. R. & Lee, S. T.(2007).Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57, 633–638.[CrossRef] [Google Scholar]
  2. Chen, Y. G., Cui, X. L., Fritze, D., Schumann, P., Pukall, R., Li, H. M., Duan, D. C., Yang, Y. L., Xu, L. H. & other authors(2007).Salinicoccus kunmingensis sp. nov., a novel halophile isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef] [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  4. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  5. Duckworth, A. W., Grant, W. D., Jones, B. E. & Steenbergen, R. V.(1996). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. França, L., Rainey, F. A., Nobre, M. F. & da Costa, M. S.(2006).Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10, 531–536.[CrossRef] [Google Scholar]
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  10. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  11. Harrigan, W. F. & McCance, M. E.(1966).Laboratory Methods in Microbiology. New York: Academic Press.
  12. Hezayen, F. F., Rehm, B. H. A., Tindall, B. J. & Steinbüchel, A.(2001). Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid). Int J Syst Evol Microbiol 51, 1133–1142.[CrossRef] [Google Scholar]
  13. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  14. Kates, M.(1986).Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier.
  15. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  16. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  17. Marquez, M. C., Ventosa, A. & Ruiz-Berraquero, F.(1990).Marinococcus hispanicus, a new species of moderately halophilic gram-positive cocci. Int J Syst Bacteriol 40, 165–169.[CrossRef] [Google Scholar]
  18. Pakdeeto, A., Tanasupawat, S., Thawai, C., Moonmangmee, S., Kudo, T. & Itoh, T.(2007).Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57, 2004–2008.[CrossRef] [Google Scholar]
  19. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  20. Sasser, M.(1990).Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  24. Ventosa, A., Marquez, M. C., Ruiz-Berraquero, F. & Kocur, M.(1990).Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13, 29–33.[CrossRef] [Google Scholar]
  25. Ventosa, A., Marquez, M. C., Weiss, N. & Tindall, B. J.(1992). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15, 530–534.[CrossRef] [Google Scholar]
  26. Zhang, W., Xue, Y., Ma, Y., Zhou, P., Ventosa, A. & Grant, W. D.(2002).Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52, 789–793.[CrossRef] [Google Scholar]
  27. Zhang, Y.-Q., Li, W.-J., Xu, L.-H. & Jiang, C.-L.(2007).Salinicoccus luteus sp. nov., a novel member of the genus Salinicoccus isolated from a desert soil. Int J Syst Evol Microbiol 57, 1901–1905.[CrossRef] [Google Scholar]
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Salinicoccus halodurans sp. nov., a moderate halophile from saline soil in China
Int J Syst Evol Microbiol 58, 1537 (2008); https://doi.org/10.1099/ijs.0.65467-0
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vol. , part 7, pp. 1537 - 1541

Transmission electron micrographs of cells of strain W24 occurring singly, in pairs and in tetrads. [PDF](342 KB)

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