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A novel species, Aspergillus uvarum sp. nov., is described within Aspergillus section Nigri. This species can be distinguished from other black aspergilli based on internal transcribed spacers (ITS), β-tubulin and calmodulin gene sequences, by AFLP analysis and by extrolite profiles. Aspergillus uvarum sp. nov. isolates produced secalonic acid, common to other Aspergillus japonicus-related taxa, and geodin, erdin and dihydrogeodin, which are not produced by any other black aspergilli. None of the isolates were found to produce ochratoxin A. The novel species is most closely related to two atypical strains of Aspergillus aculeatus, CBS 114.80 and CBS 620.78, and was isolated from grape berries in Portugal, Italy, France, Israel, Greece and Spain. The type strain of Aspergillus uvarum sp. nov. is IMI 388523T=CBS 127591T=ITEM 4834T=IBT26606T.
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 4, pp. 1032–1039
Supplementary Fig. S1. Phylogenetic tree based on ITS sequence data of Aspergillus section Nigri .
Supplementary Fig. S2. Phylogenetic tree based on calmodulin gene sequence data of Aspergillus section Nigri .
Supplementary Fig. S3. Dendrogram of representative black Aspergillus isolates together with A. uvarum isolates based on cluster analysis with the UPGMA method using the Dice genetic distance coefficient on AFLP data obtained with four primer pairs generated by the NTSYS software.