The teleomorph of was discovered through the detection of conjugation between isolates of a large collection from the nitidulid beetles of the genus found in the flowers of various cacti in Arizona, USA. The previous oversight of the sexual cycle of this yeast is attributed to the inequality (ca. 5 : 1) of the two mating types. Extensive conjugation between compatible mating types is observed after overnight incubation on 5 % malt agar, followed after 3–5 days by the formation of mature asci. The hat-shaped ascospores are reminiscent of those seen in species, which are members of the same guild. However, published analyses of D1/D2 large subunit rDNA sequences indicate an affinity with the genus . As the latter is polyphyletic and morphologically heterogeneous, and in view of the distinct life cycle of the new teleomorph, the new genus is described with a novel species, sp. nov. Given the close relatedness of sp. nov. to and , for which several natural isolates were available, strains of these species were mixed in pairs under the conditions found favourable for the former. Conjugation was not detected in those species. The type strain of sp. nov. is UWOPS 99-101.1 (=CBS 8793=NRRL Y-48386, ), type of . The allotype is UWOPS 07-123.1 (=CBS 10688=NRRL Y-48387, ).


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  1. Grant, V. & Connell, W. A.(1979). The association between Carpophilus beetles and cactus flowers. Plant Syst Evol 133, 99–102.[CrossRef] [Google Scholar]
  2. Kurtzman, C. P. & Robnett, C. J.(1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef] [Google Scholar]
  3. Kurtzman, C. P., Robnett, C. J. & Yarrow, D.(2001). Three new species of Candida from apple cider: C. anglica, C. cidri, and C. pomicola. Antonie van Leeuwenhoek 80, 237–244.[CrossRef] [Google Scholar]
  4. Lachance, M. A. & Bowles, J. M.(2002).Metschnikowia arizonensis and Metschnikowia dekortorum, two new large-spored yeast species associated with floricolous beetles. FEMS Yeast Res 2, 81–86. [Google Scholar]
  5. Lachance, M. A., Bowles, J. M., Mueller, C. & Starmer, W. T.(2000). On the biogeography of yeasts in the Wickerhamiella clade and description of Wickerhamiella lipophila sp. nov., the teleomorph of Candida lipophila. Can J Microbiol 46, 1145–1148.[CrossRef] [Google Scholar]
  6. Lachance, M. A., Bowles, J. M., Chavarria Diaz, M. M. & Janzen, D. H.(2001).Candida cleridarum, Candida tilneyi and Candida powellii, three new yeast species isolated from insects associated with flowers. Int J Syst Evol Microbiol 51, 1201–1207.[CrossRef] [Google Scholar]
  7. Lachance, M. A., Bowles, J. M. & Starmer, W. T.(2003a). Geography and niche occupancy as determinants of yeast biodiversity: the yeast-insect-morning glory ecosystem of Kı puka Puaulu, Hawai'i. FEMS Yeast Res 4, 105–111.[CrossRef] [Google Scholar]
  8. Lachance, M. A., Daniel, H. M., Meyer, W., Prasad, G. S., Gautam, S. P. & Boundy-Mills, K.(2003b). The D1/D2 domain of the large-subunit rDNA of the yeast species Clavispora lusitaniae is unusually polymorphic. FEMS Yeast Res 4, 253–258.[CrossRef] [Google Scholar]
  9. McNeill, J.(2006).International Code of Botanical Nomenclature (Vienna Code), 569 pp. Ruggell, Lichtenstein: A. R. G. Gantner Verlag KG.
  10. Nagai, S.(1963). Diagnostic color differentiation plates for hereditary respiration deficiency in yeast. J Bacteriol 86, 299–302. [Google Scholar]
  11. Nout, M. J. R. & Bartelt, R. J.(1998). Attraction of a flying nitidulid (Carpophilus humeralis) to volatiles produced by yeasts grown on sweet corn and a corn-based medium. J Chem Ecol 24, 1217–1239.[CrossRef] [Google Scholar]
  12. Werner, F. G.(1959).Carpophilus longiventris in saguaro blossoms (Coleoptera: Nitidulidae). Psyche (Stuttg) 66, 35–36.[CrossRef] [Google Scholar]
  13. Yarrow, D.(1998). Methods for the isolation and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.

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