A spore-forming, Gram-positive-staining, motile, rod-shaped and low-boron-containing bacterium was isolated from soil. The strain, designated BAM-582, can tolerate 6 % (w/v) NaCl and 50 mM boron, but optimal growth was observed without addition of boron or NaCl. The optimum temperature and pH for growth were 30 °C (range 10–37 °C) and pH 7 (range pH 6–8). A comparative analysis of the 16S rRNA gene sequence demonstrated that the isolated strain was closely related to DSM 2898 (97.7 % similarity) and IAM 13420 (98.2 %). Levels of DNA–DNA relatedness were 33.9 % with DSM 2898 and 29.5 % with DSM 28. The genomic DNA G+C content of the novel strain was 38.7 mol%. The major respiratory quinone was MK-7 and the major fatty acids were iso-C (37.4 %) and anteiso-C (19.0 %). Analysis of cell-wall amino acids revealed that the strain contained peptidoglycan with lysine, aspartic acid, alanine and glutamic acid, as is the case with other species of the genus . Based upon its distinctive peptidoglycan composition, phylogenetic and genotypic analyses and physiological characteristics, the strain BAM-582 is concluded to represent a novel species in the genus , for which the name sp. nov. is proposed (type strain BAM-582 =NBRC 103144 =KCTC 13154).


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vol. , part 6, pp. 1427 - 1432

Total boron absorption and boron concentration in cell pellets of strain BAM-582 and type strains of species of the genus

Data represent means±SD of four replications. dw, Dry weight.

Polar lipid profile of strain BAM-582 after separation by two-dimensional TLC. DPG, Diphosphatidylglycerol; NPG, ninhydrin-positive phosphoglycolipid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; L1, unknown polar lipid. Lipids were visualized by spraying the plate with iodine.

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