1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 6

sp. nov., a low-boron-containing bacterium isolated from soil Free

Abstract

A spore-forming, Gram-positive-staining, motile, rod-shaped and low-boron-containing bacterium was isolated from soil. The strain, designated BAM-582, can tolerate 6 % (w/v) NaCl and 50 mM boron, but optimal growth was observed without addition of boron or NaCl. The optimum temperature and pH for growth were 30 °C (range 10–37 °C) and pH 7 (range pH 6–8). A comparative analysis of the 16S rRNA gene sequence demonstrated that the isolated strain was closely related to DSM 2898 (97.7 % similarity) and IAM 13420 (98.2 %). Levels of DNA–DNA relatedness were 33.9 % with DSM 2898 and 29.5 % with DSM 28. The genomic DNA G+C content of the novel strain was 38.7 mol%. The major respiratory quinone was MK-7 and the major fatty acids were iso-C (37.4 %) and anteiso-C (19.0 %). Analysis of cell-wall amino acids revealed that the strain contained peptidoglycan with lysine, aspartic acid, alanine and glutamic acid, as is the case with other species of the genus . Based upon its distinctive peptidoglycan composition, phylogenetic and genotypic analyses and physiological characteristics, the strain BAM-582 is concluded to represent a novel species in the genus , for which the name sp. nov. is proposed (type strain BAM-582 =NBRC 103144 =KCTC 13154).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65455-0
2009-06-01
2021-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1427.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65455-0&mimeType=html&fmt=ahah

References

  1. Ahmed, I., Yokota, A. & Fujiwara, T. ( 2007a; ). A novel highly boron-tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. Extremophiles 11, 217–224.[CrossRef]
    [Google Scholar]
  2. Ahmed, I., Yokota, A. & Fujiwara, T. ( 2007b; ). Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57, 796–802.[CrossRef]
    [Google Scholar]
  3. Ahmed, I., Yokota, A. & Fujiwara, T. ( 2007c; ). Chimaereicella boritolerans sp. nov., a boron-tolerant and alkaliphilic bacterium of the family Flavobacteriaceae isolated from soil. Int J Syst Evol Microbiol 57, 986–992.[CrossRef]
    [Google Scholar]
  4. Ahmed, I., Yokota, A., Yamazoe, A. & Fujiwara, T. ( 2007d; ). Proposal of Lysinibacillus boronitolerans gen. nov., sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int J Syst Evol Microbiol 57, 1117–1125.[CrossRef]
    [Google Scholar]
  5. Bolaños, L., Redondo-Nieto, M., Bonilla, I. & Wall, L. G. ( 2002; ). Boron requirement in the Discaria trinervis (Rhamnaceae) and Frankia symbiotic relationship. Its essentiality for Frankia BCU110501 growth and nitrogen fixation. Physiol Plant 115, 563–570.[CrossRef]
    [Google Scholar]
  6. Bonilla, I., García-González, M. & Mateo, P. ( 1990; ). Boron requirement in cyanobacteria. Its possible role in the early evolution of photosynthetic organisms. Plant Physiol 94, 1554–1560.[CrossRef]
    [Google Scholar]
  7. Chen, X., Schauder, S., Potier, N., Dorsselaer, A. V., Pelczer, I., Bassler, B. L. & Hughson, F. M. ( 2002; ). Structural identification of a bacterial quorum-sensing signal containing boron. Nature 415, 545–549.[CrossRef]
    [Google Scholar]
  8. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  9. Çöl, M. & Çöl, C. ( 2003; ). Environmental boron contamination in waters of the Hisarcik area in the Kutahya Province of Turkey. Food Chem Toxicol 41, 1417–1420.[CrossRef]
    [Google Scholar]
  10. Cowan, S. T. ( 1974; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 2nd edn. London: Cambridge University Press.
  11. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  12. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  13. Gupta, S. K. & Stewart, J. W. B. ( 1975; ). The extraction and determination of plant-available boron in soils. Schweiz Landwirtsch Forsch 14, 153–169.
     [Google Scholar]
  14. Katsivela, E., Bonse, D., Krüger, A., Strömpl, C., Livingston, A. & Ittich, R.-M. ( 1999; ). An extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial wastewater. Appl Microbiol Biotechnol 52, 853–862.[CrossRef]
    [Google Scholar]
  15. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Kudo, T. ( 2001; ). Phospholipids. In Identification Manual of Bacteria: Molecular Genetics and Molecular Biological Methods, Springer Lab Manual, pp. 135–144. Edited by K. Suzuki, A. Hiraishi & A. Yokota. Tokyo: Springer. ISBN: 4-431-70930-4 C3045 (in Japanese).
  17. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  18. La Duc, M. T., Satomi, M. & Venkateswaran, K. ( 2004; ). Bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft. Int J Syst Evol Microbiol 54, 195–201.[CrossRef]
    [Google Scholar]
  19. Loomis, W. D. & Durst, R. W. ( 1992; ). Chemistry and biology of boron. Biofactors 3, 229–239.
     [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  22. Miwa, H., Ahmed, I., Yoon, J., Yokota, A. & Fujiwara, T. ( 2008; ). Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil. Int J Syst Evol Microbiol 58, 286–289.[CrossRef]
    [Google Scholar]
  23. Nielsen, F. H. ( 2004; ). Boron. In Elements and their Compounds in the Environment, 2nd edn, pp. 1251–1260. Edited by E. Merian, M. Anke, M. Ihnat & M. Stoeppler. Weinheim: Wiley-VCH.
  24. Otero, L., Palacio, V., Mendez, F. J. & Vazquez, F. ( 2002; ). Boric acid susceptibility testing of non-C. albicans Candida and Saccharomyces cerevisiae: comparison of three methods. Med Mycol 40, 319–322.[CrossRef]
    [Google Scholar]
  25. Priest, F. G., Goodfellow, M. & Todd, C. ( 1988; ). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882.
     [Google Scholar]
  26. Rowe, R. I. & Eckhert, C. D. ( 1999; ). Boron is required for zebrafish embryogenesis. J Exp Biol 202, 1649–1654.
     [Google Scholar]
  27. Rowe, R. I., Bouzan, C., Nabili, S. & Eckhert, C. D. ( 1998; ). The response of trout and zebrafish embryos to low and high boron concentrations is U-shaped. Biol Trace Elem Res 66, 261–270.[CrossRef]
    [Google Scholar]
  28. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
     [Google Scholar]
  29. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  30. Swate, T. E. & Weed, J. C. ( 1974; ). Boric acid treatment of vulvovaginal candidiasis. Obstet Gynecol 43, 893–895.
     [Google Scholar]
  31. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  32. Warington, K. ( 1923; ). The effect of boric acid and borax on the broad bean and certain other plants. Ann Bot (Lond) 37, 629–672.
     [Google Scholar]
  33. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
  34. Yoon, J., Maneerat, S., Kawai, F. & Yokota, A. ( 2006; ). Myroides pelagicus sp. nov., isolated from seawater in Thailand. Int J Syst Evol Microbiol 56, 1917–1920.[CrossRef]
    [Google Scholar]
  35. Yumoto, I., Hirota, K., Yamaga, S., Nodasaka, Y., Kawasaki, T., Matsuyama, H. & Nakajima, K. ( 2004; ). Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54, 1997–2001.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65455-0
Loading
Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil
Int J Syst Evol Microbiol 59, 1427 (2009); https://doi.org/10.1099/ijs.0.65455-0
/content/journal/ijsem/10.1099/ijs.0.65455-0
/content/journal/ijsem/10.1099/ijs.0.65455-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1427 - 1432

Total boron absorption and boron concentration in cell pellets of strain BAM-582 and type strains of species of the genus

Data represent means±SD of four replications. dw, Dry weight.

Polar lipid profile of strain BAM-582 after separation by two-dimensional TLC. DPG, Diphosphatidylglycerol; NPG, ninhydrin-positive phosphoglycolipid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; L1, unknown polar lipid. Lipids were visualized by spraying the plate with iodine.

IMAGE

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error