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A spore-forming, Gram-positive-staining, motile, rod-shaped and low-boron-containing bacterium was isolated from soil. The strain, designated BAM-582T, can tolerate 6 % (w/v) NaCl and 50 mM boron, but optimal growth was observed without addition of boron or NaCl. The optimum temperature and pH for growth were 30 °C (range 10–37 °C) and pH 7 (range pH 6–8). A comparative analysis of the 16S rRNA gene sequence demonstrated that the isolated strain was closely related to Lysinibacillus fusiformis DSM 2898T (97.7 % similarity) and Lysinibacillus sphaericus IAM 13420T (98.2 %). Levels of DNA–DNA relatedness were 33.9 % with L. fusiformis DSM 2898T and 29.5 % with L. sphaericus DSM 28T. The genomic DNA G+C content of the novel strain was 38.7 mol%. The major respiratory quinone was MK-7 and the major fatty acids were iso-C15 : 0 (37.4 %) and anteiso-C15 : 0 (19.0 %). Analysis of cell-wall amino acids revealed that the strain contained peptidoglycan with lysine, aspartic acid, alanine and glutamic acid, as is the case with other species of the genus Lysinibacillus. Based upon its distinctive peptidoglycan composition, phylogenetic and genotypic analyses and physiological characteristics, the strain BAM-582T is concluded to represent a novel species in the genus Lysinibacillus, for which the name Lysinibacillus parviboronicapiens sp. nov. is proposed (type strain BAM-582T =NBRC 103144T =KCTC 13154T).
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 6, pp. 1427 - 1432
Supplementary Table S1. Total boron absorption and boron concentration in cell pellets of strain BAM-582 Tand type strains of species of the genus Lysinibacillus
Data represent means±SD of four replications. dw, Dry weight.
Supplementary Fig. S1. Polar lipid profile of strain BAM-582 Tafter separation by two-dimensional TLC. DPG, Diphosphatidylglycerol; NPG, ninhydrin-positive phosphoglycolipid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; L1, unknown polar lipid. Lipids were visualized by spraying the plate with iodine.