sp. nov., isolated from a polycyclic aromatic hydrocarbon-contaminated soil Free

Abstract

A Gram-negative, rod-shaped, aerobic bacterium, designated strain RP007, was isolated from a polycyclic aromatic hydrocarbon-contaminated soil in New Zealand. Two additional strains were recovered from a compost heap in Belgium (LMG 18808) and from the rhizosphere of maize in the Netherlands (LMG 24204). The three strains had virtually identical 16S rRNA gene sequences and whole-cell protein profiles, and they were identified as members of the genus , with as their closest relative. Strain RP007 had a DNA G+C content of 63.5 mol% and could be distinguished from based on a range of biochemical characteristics. Strain RP007 showed levels of DNA–DNA relatedness towards the type strain of and those of other recognized species of less than 30 %. The results of 16S rRNA gene sequence analysis, DNA–DNA hybridization experiments and physiological and biochemical tests allowed the differentiation of strain RP007 from all recognized species of the genus . Strains RP007, LMG 18808 and LMG 24204 are therefore considered to represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is RP007 (=LMG 24000 =CCUG 53604 =ICMP 13529).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65451-0
2008-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/420.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65451-0&mimeType=html&fmt=ahah

References

  1. Bogardt, A. H. & Hemmingsen, B. B.(1992). Enumeration of phenanthrene-degrading bacteria by an overlayer technique and its use in evaluation of petroleum-contaminated sites. Appl Environ Microbiol 58, 2579–2582. [Google Scholar]
  2. Coenye, T. & Vandamme, P.(2003). Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5, 719–729.[CrossRef] [Google Scholar]
  3. Coenye, T., Laevens, S., Willems, A., Ohlén, M., Hannant, W., Govan, J. R., Gillis, M., Falsen, E. & Vandamme, P.(2001).Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol 51, 1099–1107.[CrossRef] [Google Scholar]
  4. Coenye, T., Vanlaere, E., Samyn, E., Falsen, E., Larsson, P. & Vandamme, P.(2005).Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples. Int J Syst Evol Microbiol 55, 251–256.[CrossRef] [Google Scholar]
  5. Kim, H. B., Park, M. J., Yang, H. C., An, D. S., Jin, H. Z. & Yang, D. C.(2006).Burkholderia ginsengisoli sp. nov., a β-glucosidase-producing bacterium isolated from soil of a ginseng field. Int J Syst Evol Microbiol 56, 2529–2533.[CrossRef] [Google Scholar]
  6. Laurie, A. D. & Lloyd-Jones, G.(1999). The phn genes of Burkholderia sp. strain RP007 constitute a divergent gene cluster for polycyclic aromatic hydrocarbon catabolism. J Bacteriol 181, 531–540. [Google Scholar]
  7. Mesbah, M. & Whitman, W. B.(1989). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef] [Google Scholar]
  8. O'Sullivan, L. A. & Mahenthiralingam, E.(2005). Biotechnological potential within the genus Burkholderia. Lett Appl Microbiol 41, 8–11.[CrossRef] [Google Scholar]
  9. Parke, J. L. & Gurian-Sherman, D.(2001). Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annu Rev Phytopathol 39, 225–258.[CrossRef] [Google Scholar]
  10. Pitcher, D. G., Saunders, N. A. & Owen, R. J.(1989). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef] [Google Scholar]
  11. Pot, B., Vandamme, P. & Kersters, K.(1994). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  12. Salles, J. F., Samyn, E., Vandamme, P., Van Veen, J. A. & Van Elsas, J. D.(2006). Changes in agricultural management drive the diversity of Burkholderia species isolated from soil on PCAT medium. Soil Biol Biochem 38, 661–673.[CrossRef] [Google Scholar]
  13. Tecon, R., Wells, M. & Van Der Meer, J. R.(2006). A new green fluorescent protein-based bacterial biosensor for analysing phenanthrene fluxes. Environ Microbiol 8, 697–708.[CrossRef] [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  15. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R. & other authors(1992). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef] [Google Scholar]
  16. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J.(1996). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438. [Google Scholar]
  17. Viallard, V., Poirier, I., Cournoyer, B., Haurat, J., Wiebkin, S., Ophel-Keller, K. & Balandreau, J.(1998).Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia. Int J Syst Bacteriol 48, 549–563.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65451-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65451-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed