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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 10

gen. nov., sp. nov., a novel marine bacterium of the family Free

Abstract

A novel aerobic, non-motile, Gram-negative bacterium, designated AS06/20a, was isolated from coastal waters of the Adriatic Sea and subjected to polyphasic taxonomic analysis. Cells were rod-shaped and formed non-pigmented punctiform colonies on agar plates. The novel isolate grew heterotrophically on a variety of carbon compounds, including organic acids, carbohydrates, polyols, amino acids and complex organic substrates. Cells grew at 15–33 °C (optimum 30 °C), pH 6.5–8.5 (optimum pH 7.5–8.0) and between 2 and 6 % (w/v) NaCl (optimum 2 %). The dominant fatty acids (>5 %) detected in strain AS06/20a were iso-C G, iso-C, iso-C 3-OH, iso-C 9 and iso-C 3-OH. The major respiratory quinone was MK-6. The G+C content of the genomic DNA was 38.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AS06/20a fell within the family in the phylum . Comparative 16S rRNA gene sequence analysis indicated that strain AS06/20a was most closely related to members of the genera and , sharing 92–93 % 16S rRNA gene sequence similarity with representatives of these genera. It is proposed, from the results of the polyphasic taxonomic analysis, that the strain should be placed in a new genus, gen. nov., with sp. nov. as the type species. The type strain is AS06/20a (=DSM 19308=CIP 109577=OOB 358).

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Keyword(s): ASW, artificial seawater , DOM, dissolved organic material and HMM, high molecular mass
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2008-10-01
2025-03-23
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References

  1. Agogué, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., Herndl, G. J. & Lebaron, P.(2005). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54, 269–280.[CrossRef] [Google Scholar]
  2. Alain, K., Querellou, J., Lesongeur, F., Pignet, P., Crassous, P., Raguénès, G., Cueff, V. & Cambon-Bonavita, M.-A.(2002).Caminibacter hydrogeniphilus gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52, 1317–1323.[CrossRef] [Google Scholar]
  3. Alonso, C. & Pernthaler, J.(2005). Incorporation of glucose under anoxic conditions by bacterioplankton from coastal North Sea surface waters. Appl Environ Microbiol 71, 1709–1716.[CrossRef] [Google Scholar]
  4. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  5. Amon, R. M. W. & Benner, R.(1996). Bacterial utilization of different size classes of dissolved organic matter. Limnol Oceanogr 41, 41–51.[CrossRef] [Google Scholar]
  6. Asker, D., Beppu, T. & Ueda, K.(2007).Zeaxanthinibacter enoshimensis gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enosima Island, Japan. Int J Syst Evol Microbiol 57, 837–843.[CrossRef] [Google Scholar]
  7. Barbeyron, T., L'Haridon, S., Corre, E., Kloareg, B. & Potin, P.(2001).Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (Zobell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 985–997.[CrossRef] [Google Scholar]
  8. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  9. Bernardet, J.-F., Nakagawa, Y. & Holmes, B., for the Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  10. Cho, J.-C. & Giovannoni, S. J.(2004).Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content. Int J Syst Evol Microbiol 54, 1101–1106.[CrossRef] [Google Scholar]
  11. Cole, J. J., Findlay, S. & Pace, M. L.(1988). Bacterioplanktonic production in fresh and salt-water ecosystems: a crossover view. Mar Ecol Prog Ser 43, 1–10.[CrossRef] [Google Scholar]
  12. Cottrell, M. T. & Kirchman, D. L.(2000a). Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66, 5116–5122.[CrossRef] [Google Scholar]
  13. Cottrell, M. T. & Kirchman, D. L.(2000b). Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl Environ Microbiol 66, 1692–1697.[CrossRef] [Google Scholar]
  14. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  15. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  16. Galtier, N., Gouy, M. & Gautier, C.(1996).seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548. [Google Scholar]
  17. Glöckner, F. O., Fuchs, B. M. & Amann, R.(1999). Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65, 3721–3726. [Google Scholar]
  18. Ivanova, E. P., Nedashkovskaya, O. I., Chun, J., Lysenko, A. M., Frolova, G. M., Svetashev, V. I., Vysotskii, M. V., Mikhailov, V. V., Huq, A. & Colwell, R. R.(2001).Arenibacter gen. nov., a new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51, 1987–1995.[CrossRef] [Google Scholar]
  19. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  20. Khan, S. T., Nakagawa, Y. & Harayama, S.(2006).Sediminicola luteus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 56, 841–845.[CrossRef] [Google Scholar]
  21. Kirchman, D. L.(2002). The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39, 91–100. [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  23. MIDI(2001).Sherlock Microbial Identification System. Newark, DE: MIDI.
  24. Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Lysenko, A. M., Rohde, M., Rhee, M.-S., Frolova, G. M., Falsen, E., Mikhailov, V. V. & Bae, K. S.(2004).Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54, 1017–1023.[CrossRef] [Google Scholar]
  25. Nedashkovskaya, O. I., Kim, S. B., Lee, K. H., Bae, K. S., Frolova, G. M., Mikhailov, V. V. & Kim, I. S.(2005).Pibocella ponti gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi. Int J Syst Evol Microbiol 55, 177–181.[CrossRef] [Google Scholar]
  26. Raguénès, G., Christen, R., Guezennec, J., Pignet, P. & Barbier, G.(1997).Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef] [Google Scholar]
  27. Reichenbach, H.(1989). The order Cytophagales Leadbetter 1974, 99AL. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  28. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  29. Simon, M., Glöckner, F. O. & Amann, R.(1999). Different community structure and temperature optima of heterotrophic picoplankton in various regions of the Southern Ocean. Aquat Microb Ecol 18, 275–284.[CrossRef] [Google Scholar]
  30. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  31. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
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Eudoraea adriatica gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae
Int J Syst Evol Microbiol 58, 2275 (2008); https://doi.org/10.1099/ijs.0.65446-0
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Transmission electron micrograph of cells of strain AS06/20a ( gen. nov., sp. nov.) in a state of division. [PDF](162 KB)

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Graphs showing the effects of temperature (a), pH (b) and salinity (c) on the maximum growth rate of strain AS06/20a ( gen. nov., sp. nov.). [PDF](23 KB)

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