A novel aerobic, non-motile, Gram-negative bacterium, designated AS06/20a, was isolated from coastal waters of the Adriatic Sea and subjected to polyphasic taxonomic analysis. Cells were rod-shaped and formed non-pigmented punctiform colonies on agar plates. The novel isolate grew heterotrophically on a variety of carbon compounds, including organic acids, carbohydrates, polyols, amino acids and complex organic substrates. Cells grew at 15–33 °C (optimum 30 °C), pH 6.5–8.5 (optimum pH 7.5–8.0) and between 2 and 6 % (w/v) NaCl (optimum 2 %). The dominant fatty acids (>5 %) detected in strain AS06/20a were iso-C G, iso-C, iso-C 3-OH, iso-C 9 and iso-C 3-OH. The major respiratory quinone was MK-6. The G+C content of the genomic DNA was 38.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AS06/20a fell within the family in the phylum . Comparative 16S rRNA gene sequence analysis indicated that strain AS06/20a was most closely related to members of the genera and , sharing 92–93 % 16S rRNA gene sequence similarity with representatives of these genera. It is proposed, from the results of the polyphasic taxonomic analysis, that the strain should be placed in a new genus, gen. nov., with sp. nov. as the type species. The type strain is AS06/20a (=DSM 19308=CIP 109577=OOB 358).


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Transmission electron micrograph of cells of strain AS06/20a ( gen. nov., sp. nov.) in a state of division. [PDF](162 KB)


Graphs showing the effects of temperature (a), pH (b) and salinity (c) on the maximum growth rate of strain AS06/20a ( gen. nov., sp. nov.). [PDF](23 KB)

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