RT Journal Article SR Electronic(1) A1 Chen, Yi-Guang A1 Cui, Xiao-Long A1 Kroppenstedt, Reiner M. A1 Stackebrandt, Erko A1 Wen, Meng-Liang A1 Xu, Li-Hua A1 Jiang, Cheng-LinYR 2008 T1 Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 3 SP 699 OP 705 DO https://doi.org/10.1099/ijs.0.65441-0 PB Microbiology Society, SN 1466-5034, AB A previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4T, was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, and was investigated using a polyphasic taxonomic approach. The strain grew well on most of the media tested, producing white to pale-yellow substrate mycelium, white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight spore chains. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. No diffusible pigments were produced on any of the media tested. The strain grew in the presence of 0–10 % (w/v) NaCl and at pH 6.0–8.0, with optimum growth occurring at 3 % (w/v) NaCl and pH 7.0. It grew at 10–37 °C, the optimum growth temperature being 28 °C. Whole-cell hydrolysates of strain YIM 28A4T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinones were MK-10, MK-10(H2), MK-11 and MK-11(H2). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 67.1 mol%. The morphological and chemotaxonomic characteristics of the isolate matched those described for Nocardiopsis species. A phylogenetic analysis based on 16S rRNA gene sequence comparisons confirmed that strain YIM 28A4T was a member of the genus Nocardiopsis and most closely related to the type strains Nocardiopsis aegyptia DSM 44442T and Nocardiopsis halotolerans DSM 44410T, showing 98.1 and 97.8 % 16S rRNA gene sequence similarity, respectively. Strain YIM 28A4T can be differentiated from these type strains by using phenotypic, phylogenetic and DNA–DNA hybridization data. On the basis of the polyphasic evidence, strain YIM 28A4T represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis quinghaiensis sp. nov. is proposed. The type strain is YIM 28A4T (=DSM 44739T =CGMCC 4.3494T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65441-0