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Abstract

A novel bacterial strain, designated a4, isolated from a sea anemone () in Taiwan, was characterized using a polyphasic taxonomic approach. Strain a4 was aerobic, Gram-negative, pale-yellow-pigmented and rod-shaped. It grew optimally at 30–35 °C, in the presence of 3–4 % (w/v) NaCl and at pH 8.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus (family , phylum ). The closest neighbours were TF-26 (97.6 % similarity) and SMK-4 (97.7 % similarity). The novel isolate could be distinguished from all species by several phenotypic characteristics. The major fatty acids were summed feature 3 (comprising C 7 and/or iso-C 2-OH, 19.6 %), iso-C (12.9 %), iso-C 3-OH (10.2 %), iso-C 3-OH (9.9 %) and iso-C (9.5 %). The DNA G+C content was 35.0 mol%. Hence, genotypic and phenotypic data demonstrate that strain a4 should be classified as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is a4 (=BCRC 17655 =LMG 24004).

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2008-04-01
2022-01-21
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References

  1. Bernardet, J.-F. & Nakagawa, Y.(2006). An introduction to the family Flavobacteriaceae. In The Prokaryotes, 3rd edn, vol. 7, pp. 455–480. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  2. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  4. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  5. Chang, S. C., Wang, J. T., Vandamme, P., Hwang, J. H., Chang, P. S. & Chen, W. M.(2004).Chitinimonas taiwanensis gen. nov., sp. nov., a novel chitinolytic bacterium isolated from a freshwater pond for shrimp culture. Syst Appl Microbiol 27, 43–49.[CrossRef] [Google Scholar]
  6. Chen, W. M., Laevens, S., Lee, T. M., Coenye, T., De Vos, P., Mergeay, M. & Vandamme, P.(2001).Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51, 1729–1735.[CrossRef] [Google Scholar]
  7. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H. & Chun, J.Cho, B. C.(2006).Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef] [Google Scholar]
  8. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  9. Collins, M. D., Hutson, R. A., Båverud, V. & Falsen, E.(2000). Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov. Int J Syst Evol Microbiol 50, 1247–1251.[CrossRef] [Google Scholar]
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  11. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N.(2004).Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef] [Google Scholar]
  12. GCG(1995).Wisconsin Package Version 8.1 Program Manual. Madison, WI: Genetics Computer Group.
  13. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  14. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  15. Hansen, G. H., Bergh, Ø., Michaelsen, J. & Knappskog, D.(1992).Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42, 451–458.[CrossRef] [Google Scholar]
  16. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  17. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H.(2006).Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef] [Google Scholar]
  18. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  19. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  20. Lyman, J. & Fleming, R. H.(1940). Composition of sea water. J Mar Res 3, 134–146. [Google Scholar]
  21. MacFaddin, J. F.(2000).Biochemical Tests for the Identification of Medical Bacteria, 3rd edn. Baltimore: Lippincott Williams & Wilkins.
  22. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  23. Piñeiro-Vidal, M., Riaza, A. & Santos, Y.(2008).Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58, 21–25.[CrossRef] [Google Scholar]
  24. Powers, E. M.(1995). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758. [Google Scholar]
  25. Reichenbach, H.(1992a). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  26. Reichenbach, H.(1992b).Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42, 327–328.[CrossRef] [Google Scholar]
  27. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  28. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Sheu, S. Y., Lin, K. Y., Chou, J. H., Chang, P. S., Arun, A. B., Young, C. C. & Chen, W. M.(2007).Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef] [Google Scholar]
  30. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S.(2001). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef] [Google Scholar]
  31. Wakabayashi, H., Hikida, M. & Masumura, K.(1986).Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36, 396–398.[CrossRef] [Google Scholar]
  32. Wang, J. T. & Douglas, A. E.(1997). Nutrients, signals, and photosynthate release by symbiotic algae: the impact of taurine on the dinoflagellate alga Symbiodinium from the sea anemone Aiptasia pulchella. Plant Physiol 114, 631–636. [Google Scholar]
  33. Wang, J. T. & Douglas, A. E.(1998). Nitrogen recycling or nitrogen conservation in an alga-invertebrate symbiosis? J Exp Biol 201, 2445–2453. [Google Scholar]
  34. Wang, J. T. & Douglas, A. E.(1999). Essential amino acid synthesis and nitrogen recycling in an alga-invertebrate symbiosis. Mar Biol 135, 219–222.[CrossRef] [Google Scholar]
  35. Yoon, J. H., Kang, S. J. & Oh, T. K.(2005).Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef] [Google Scholar]
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[PDF file of Supplementary Tables S1 and S2](137 KB)

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Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences of strain a4 and other species, including two species described after this paper was accepted for publication. [PDF](405 KB)

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