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Abstract

A novel bacterial strain, designated a4, isolated from a sea anemone () in Taiwan, was characterized using a polyphasic taxonomic approach. Strain a4 was aerobic, Gram-negative, pale-yellow-pigmented and rod-shaped. It grew optimally at 30–35 °C, in the presence of 3–4 % (w/v) NaCl and at pH 8.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus (family , phylum ). The closest neighbours were TF-26 (97.6 % similarity) and SMK-4 (97.7 % similarity). The novel isolate could be distinguished from all species by several phenotypic characteristics. The major fatty acids were summed feature 3 (comprising C 7 and/or iso-C 2-OH, 19.6 %), iso-C (12.9 %), iso-C 3-OH (10.2 %), iso-C 3-OH (9.9 %) and iso-C (9.5 %). The DNA G+C content was 35.0 mol%. Hence, genotypic and phenotypic data demonstrate that strain a4 should be classified as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is a4 (=BCRC 17655 =LMG 24004).

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2008-04-01
2020-01-26
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[PDF file of Supplementary Tables S1 and S2](137 KB)

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Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences of strain a4 and other species, including two species described after this paper was accepted for publication. [PDF](405 KB)

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