1887

Abstract

A Gram-negative, moderately halophilic bacterium, designated YIM 91125, was isolated from a salt lake in Xinjiang province, north-west China. The isolate grew at salinities in the range 1–20 % (w/v) and at 4–45 °C. Optimal growth occurred at 37 °C, pH 7.5 and 5–10 % (w/v) NaCl. Cells were short rods motile by means of single polar flagella. The major fatty acids were C 7, C, C cyclo 8 and C 3-OH. The DNA G+C content was 60.8 mol%. The predominant respiratory quinone was Q-9. A comparison of 16S rRNA gene sequences revealed its relationship to species, its closest neighbours being (95.9 % similarity to the type strain) and (95.4 % similarity). On the basis of chemotaxonomic, phylogenetic and phenotypic evidence, strain YIM 91125 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is YIM 91125 (=KCTC 12847 =CCTCC AB 206093).

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2008-09-01
2020-01-20
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References

  1. Arahal, D. R., Castillo, A. M., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002a; ). Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst Appl Microbiol 25, 207–211.[CrossRef]
    [Google Scholar]
  2. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002b; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  3. Berendes, F., Gottschalk, G., Heine-Dobbernack, E., Moore, E. R. B. & Tindall, B. J. ( 1996; ). Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 19, 158–167.[CrossRef]
    [Google Scholar]
  4. Cabrera, A., Aguilera, M., Fuentes, S., Incerti, C., Russell, N. J., Ramos-Cormenzana, A. & Monteoliva-Sánchez, M. ( 2007; ). Halomonas indalinina sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almería, southern Spain. Int J Syst Evol Microbiol 57, 376–380.[CrossRef]
    [Google Scholar]
  5. Cerny, G. ( 1978; ). Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 113–122.[CrossRef]
    [Google Scholar]
  6. Cui, H.-L., Yang, Y., Tohty, D., Zhou, P.-J. & Liu, S.-J. ( 2006; ). Biodiversity of halophilic archaea isolated from two salt lakes in Xin-Jiang region of China. Wei Sheng Wu Xue Bao 46, 171–175 (in Chinese).
    [Google Scholar]
  7. Dobson, S. J. & Franzmann, P. D. ( 1996; ). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef]
    [Google Scholar]
  8. Drews, G. ( 1983; ). Mikrobiologisches Praktikum, 4th edn. Berlin: Springer (in German).
  9. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  11. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  12. Franzmann, P. D., Wehmeyer, U. & Stackebrandt, E. ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11, 16–19.[CrossRef]
    [Google Scholar]
  13. Heyrman, J., Balcaen, A., De Vos, P. & Swings, J. ( 2002; ). Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 52, 2049–2054.[CrossRef]
    [Google Scholar]
  14. Jeon, C. O., Kim, J. M., Lee, J. R., Lee, G. S., Park, D. J., Lee, J. C., Oh, H. W. & Kim, C. J. ( 2007; ). Halomonas kribbensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 57, 2194–2198.[CrossRef]
    [Google Scholar]
  15. Kim, K. K., Jin, L., Yang, H. C. & Lee, S. T. ( 2007; ). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. Int J Syst Evol Microbiol 57, 675–681.[CrossRef]
    [Google Scholar]
  16. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  17. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  18. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  19. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  20. Leifson, E. ( 1960; ). Atlas of Bacterial Flagellation. London: Academic Press.
  21. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  22. Margesin, R. & Schinner, F. ( 2001; ). Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5, 73–83.[CrossRef]
    [Google Scholar]
  23. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  24. Mata, J. A., Martínez-Cánovas, J., Quesada, E. & Béjar, V. ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  25. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef]
    [Google Scholar]
  26. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  27. Reva, O. N., Vyunitskaya, V. A., Reznik, S. R., Kozachko, A. & Smirnov, V. V. ( 1995; ). Antibiotic susceptibility as a taxonomic characteristic of the genus Bacillus. Int J Syst Bacteriol 45, 409–441.[CrossRef]
    [Google Scholar]
  28. Romano, I., Nicolaus, B., Lama, L., Manca, M. C. & Gambacorta, A. ( 1997; ). Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria Island. Int J Syst Bacteriol 47, 601–602.[CrossRef]
    [Google Scholar]
  29. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  30. Sánchez-Porro, C., Martín, S., Mellado, E. & Ventosa, A. ( 2003; ). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J Appl Microbiol 94, 295–300.[CrossRef]
    [Google Scholar]
  31. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  32. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  33. Soto-Ramírez, N., Sánchez-Porro, C., Rosas, S., González, W., Quiñones, M., Ventosa, A. & Montalvo-Rodríguez, R. ( 2007; ). Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico. Int J Syst Evol Microbiol 57, 900–905.[CrossRef]
    [Google Scholar]
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  35. Ventosa, A. & Nieto, J. J. ( 1995; ). Biotechnological applications and potentialities of halophilic microorganisms. World J Microbiol Biotechnol 11, 85–94.[CrossRef]
    [Google Scholar]
  36. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  37. Wang, Y. N., Cai, H., Yu, S. L., Wang, Z. Y., Liu, J. & Wu, X. L. ( 2007a; ). Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 57, 911–915.[CrossRef]
    [Google Scholar]
  38. Wang, Y. N., Cai, H., Chi, C. Q., Lu, A. H., Lin, X. G., Jiang, Z. F. & Wu, X. L. ( 2007b; ). Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium. Int J Syst Evol Microbiol 57, 1222–1226.[CrossRef]
    [Google Scholar]
  39. Xu, P., Li, W. J., Tang, S. K., Zhang, Y. Q., Chen, G. Z., Chen, H. H., Xu, L. H. & Jiang, C. L. ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55, 1149–1153.[CrossRef]
    [Google Scholar]
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