%0 Journal Article %A Chou, Jui-Hsing %A Jiang, Sing-Rong %A Cho, Jang-Cheon %A Song, Jaeho %A Lin, Mei-Chun %A Chen, Wen-Ming %T Azonexus hydrophilus sp. nov., a nifH gene-harbouring bacterium isolated from freshwater %D 2008 %J International Journal of Systematic and Evolutionary Microbiology, %V 58 %N 4 %P 946-951 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65434-0 %I Microbiology Society, %X Three Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1T, d8-2 and IMCC1716, were isolated from a freshwater spring sample and a eutrophic freshwater pond. Based on characterization using a polyphasic approach, the three strains showed highly similar phenotypic, physiological and genetic characteristics. All of the strains harboured the nitrogenase gene nifH, but nitrogen-fixing activities could not be detected in nitrogen-free culture media. The three strains shared 99.6–99.7 % 16S rRNA gene sequence similarity and showed 89–100 % DNA–DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains d8-1T, d8-2 and IMCC1716 formed a monophyletic branch in the periphery of the evolutionary radiation occupied by the genus Azonexus. Their closest neighbours were Azonexus caeni Slu-05T (96.7–96.8 % similarity) and Azonexus fungiphilus BS5-8T (96.3–96.6 %). The DNA–DNA relatedness of the novel strains to these two species of the genus Azonexus was less than 70 %. The isolates could also be differentiated from recognized members of the genus Azonexus on the basis of phenotypic and biochemical characteristics. It is evident, therefore, that the three strains represent a novel species of the genus Azonexus, for which the name Azonexus hydrophilus sp. nov. is proposed. The type strain is d8-1T (=LMG 24005T=BCRC 17657T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65434-0