@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65434-0, author = "Chou, Jui-Hsing and Jiang, Sing-Rong and Cho, Jang-Cheon and Song, Jaeho and Lin, Mei-Chun and Chen, Wen-Ming", title = "Azonexus hydrophilus sp. nov., a nifH gene-harbouring bacterium isolated from freshwater", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "4", pages = "946-951", doi = "https://doi.org/10.1099/ijs.0.65434-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65434-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1T, d8-2 and IMCC1716, were isolated from a freshwater spring sample and a eutrophic freshwater pond. Based on characterization using a polyphasic approach, the three strains showed highly similar phenotypic, physiological and genetic characteristics. All of the strains harboured the nitrogenase gene nifH, but nitrogen-fixing activities could not be detected in nitrogen-free culture media. The three strains shared 99.6–99.7 % 16S rRNA gene sequence similarity and showed 89–100 % DNA–DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains d8-1T, d8-2 and IMCC1716 formed a monophyletic branch in the periphery of the evolutionary radiation occupied by the genus Azonexus. Their closest neighbours were Azonexus caeni Slu-05T (96.7–96.8 % similarity) and Azonexus fungiphilus BS5-8T (96.3–96.6 %). The DNA–DNA relatedness of the novel strains to these two species of the genus Azonexus was less than 70 %. The isolates could also be differentiated from recognized members of the genus Azonexus on the basis of phenotypic and biochemical characteristics. It is evident, therefore, that the three strains represent a novel species of the genus Azonexus, for which the name Azonexus hydrophilus sp. nov. is proposed. The type strain is d8-1T (=LMG 24005T=BCRC 17657T).", }