A Gram-negative, heterotrophic, aerobic, reddish-orange-pigmented, non-spore-forming, non-motile bacterial strain, designated 7-UAH, was isolated from salty water from the athalassohaline lagoon at El Hito, located in central Spain. The strain grew optimally at 37 °C and pH 7.0 and in the presence of 2.5 % NaCl. A polyphasic taxonomic study was carried out in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain 7-UAH clustered within the branch constituted by species of the genus , which were recently transferred to the genus . Analysis of the polar lipid profile and DNA G+C content also supported placement of strain 7-UAH within the genus . On the basis of its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 7-UAH represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 7-UAH (=CECT 7267 =CCM 7449).


Article metrics loading...

Loading full text...

Full text loading...



  1. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M.(1966). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496. [Google Scholar]
  2. Bowman, J. P., Nichols, C. M. & Gibson, J. A. E.(2003).Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef] [Google Scholar]
  3. Christensen, W. B.(1946). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466. [Google Scholar]
  4. Cowan, S. T. & Steel, K. J.(1974).Manual for the Identification of Medical Bacteria. Cambridge: Cambridge University Press.
  5. Ensor, L., Stosz, S. & Weiner, R.(1999). Expression of multiple complex polysaccharide-degrading enzyme systems by marine bacterium strain 2-40. J Ind Microbiol Biotechnol 23, 123–126.[CrossRef] [Google Scholar]
  6. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  7. Huang, X. & Madan, A.(1999).cap3: a DNA sequence assembly program. Genome Res 9, 868–877.[CrossRef] [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  9. Kovács, N.(1956). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178, 703–704. [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  12. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  13. Miller, L. T.(1982). Single derivation method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  14. Nedashkovskaya, O. I., Vancanneyt, M., Van Trappen, S., Vandemeulebroecke, K., Lysenko, A. M., Rhode, M., Falsen, E., Frolova, G. M., Mikhailov, V. V. & other authors(2004). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 54, 1757–1764.[CrossRef] [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Kwon, K. K., Shin, D. S., Luo, X., Kim, S.-J. & Mikhailov, V. V.(2007). Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57, 1988–1994.[CrossRef] [Google Scholar]
  16. Owen, R. J. & Hill, L. R.(1979). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Chemical Methods for Microbiologists, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  17. Reichenbach, H.(1989). Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  18. Reysenbach, A. L., Longnecker, K. & Kirshtein, J.(2000). Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a mid-Atlantic ridge hydrothermal vent. Appl Environ Microbiol 66, 3798–3806.[CrossRef] [Google Scholar]
  19. Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Comenzana, A.(1981). Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol 7, 235–243.[CrossRef] [Google Scholar]
  20. Sawabe, T., Makino, H., Tatsumi, M., Nakano, K., Tajima, K., Iqbal, M. M., Yumoto, I., Ezura, Y. & Christen, R.(1998).Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica. Int J Syst Bacteriol 48, 769–774.[CrossRef] [Google Scholar]
  21. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Tiago, I., Mendes, V., Pires, C., Morais, P. V. & Verissimo, A.(2006).Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29, 100–108.[CrossRef] [Google Scholar]
  24. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J.(2004).Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 1969–1973.[CrossRef] [Google Scholar]
  25. Yi, H. & Chun, J.(2004).Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 157–162.[CrossRef] [Google Scholar]
  26. Yoon, J.-H., Yeo, S.-H. & Oh, T.-K.(2004).Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54, 1845–1848.[CrossRef] [Google Scholar]
  27. Yoon, J.-H., Kang, S.-J., Jung, S.-Y., Lee, C.-H. & Oh, T.-K.(2005a).Algoriphagus yeomjeomi sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 865–870.[CrossRef] [Google Scholar]
  28. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2005b).Algoriphagus locisalis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 55, 1635–1639.[CrossRef] [Google Scholar]
  29. Yoon, J.-H., Lee, M.-H., Kang, S.-J. & Oh, T.-K.(2006).Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 777–780.[CrossRef] [Google Scholar]

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error