A moderately halophilic Gram-negative bacterium, strain DS2, was isolated from seawater from the East Sea in Korea. Strain DS2 grew at salinities of 0–14 % (w/v) NaCl and at temperatures in the range 10–38 °C. The cells were non-motile short rods (1.0–1.4 μm in width and 1.6–2.6 μm in length) and the major fatty acids were C 7 and 11-methyl C 7. The genomic DNA G+C content was 72.0 mol% and the predominant lipoquinone was Q-10. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS2 formed a distinct phyletic line from the genus within the . The levels of 16S rRNA gene sequence similarity with respect to the type strains of related genera were below 94 %. On the basis of physiological and phylogenetic properties, strain DS2 represents a novel genus and species of the , for which the name gen. nov., sp. nov. is proposed. The type strain is DS2 (=KCTC 12586=DSM 17890).


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  1. Albuquerque, L., Santos, J., Travassos, P., Nobre, M. F., Rainey, F. A., Wait, R., Empadinhas, N., Silva, M. T. & da Costa, M. S.(2002).Albidovulum inexpectatum gen. nov., sp. nov., a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus Rhodovulum. Appl Environ Microbiol 68, 4266–4273.[CrossRef] [Google Scholar]
  2. Biebl, H., Pukall, R., Lünsdorf, H., Schulz, S., Allgaier, M., Tindall, B. J. & Wagner-Döbler, I.(2007). Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium. Int J Syst Evol Microbiol 57, 1095–1107.[CrossRef] [Google Scholar]
  3. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Distributed by author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  6. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  7. Hiraishi, A. & Ueda, Y.(1995). Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int J Syst Bacteriol 45, 319–326.[CrossRef] [Google Scholar]
  8. Imhoff, J. F., Trüper, H. G. & Pfennig, N.(1984). Rearrangement of the species and genera of the phototrophic “purple nonsulfur bacteria”. Int J Syst Bacteriol 34, 340–343.[CrossRef] [Google Scholar]
  9. Jeon, C. O., Lim, J.-M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C.-J.(2005). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef] [Google Scholar]
  10. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  11. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  12. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  13. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  14. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  15. Sato, K.(1978). Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1. FEBS Lett 85, 207–210.[CrossRef] [Google Scholar]
  16. Sorokin, D. Yu., Tourova, T. P., Spiridonova, E. M., Rainey, F. A. & Muyzer, G.(2005).Thioclava pacifica gen. nov., sp. nov., a novel facultatively autotrophic, marine, sulfur-oxidizing bacterium from a near-shore sulfidic hydrothermal area. Int J Syst Evol Microbiol 55, 1069–1075.[CrossRef] [Google Scholar]
  17. Suzuki, T., Muroga, Y., Takahama, M., Shiba, T. & Nishimura, Y.(1999).Rubrimonas cliftonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium isolated from a saline lake. Int J Syst Bacteriol 49, 201–205.[CrossRef] [Google Scholar]
  18. Suzuki, T., Mori, Y. & Nishimura, Y.(2006).Roseibacterium elongatum gen. nov., sp. nov., an aerobic, bacteriochlorophyll-containing bacterium isolated from the west coast of Australia. Int J Syst Evol Microbiol 56, 417–421.[CrossRef] [Google Scholar]
  19. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]

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vol. , part 1, pp. 282 - 285

Transmission electron micrograph of negatively stained cells of strain DS2 ( gen. nov., sp. nov.). [PDF](95 KB)


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