Samples from plants of exhibiting typical witches'-broom symptoms ( witches'-broom; CWB) were examined for the presence of plant pathogenic phytoplasmas by PCR amplification using universal phytoplasma primers. All affected plants yielded positive results. RFLP analyses of rRNA gene products indicated that the phytoplasmas detected were different from those described previously. Phylogenetic analysis of 16S rRNA gene sequences confirmed that CWB represents a distinct lineage and shares a common ancestor with ‘ Phytoplasma phoenicium’. Molecular comparison revealed that the 16S rRNA gene sequences of the four CWB strains (IM-1, IM-2, IM-3 and IM-4) identified in symptomatic samples were nearly identical (99.6–100 % similarity). The closest relatives were members of the pigeon pea witches'-broom phytoplasma ribosomal group (16SrIX; 95–97 % sequence similarity). On the basis of unique 16S rRNA gene sequences and biological properties, the phytoplasma associated with witches'-broom of in Oman represents a coherent but discrete novel phytoplasma, ‘ Phytoplasma omanense’, with GenBank/DDBJ/EMBL accession number EF666051 representing the reference strain.


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  1. Abou-Jawdah, Y., Karakashian, A., Sobh, H., Martini, M. & Lee, I.-M.(2003). An epidemic of almond witches'-broom in Lebanon: classification and phylogenetic relationships of the associated phytoplasmas. Plant Dis 86, 477–484. [Google Scholar]
  2. Ahrens, U. & Seemüller, E.(1992). Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828–832.[CrossRef] [Google Scholar]
  3. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  4. Arocha, Y., López, M., Piñol, B., Fernández, M., Picornell, B., Almeida, R., Palenzuela, I., Wilson, M. R. & Jones, P.(2005).Candidatus Phytoplasma graminis’ and ‘Candidatus Phytoplasma caricae’, two novel phytoplasmas associated with diseases of sugarcane, weeds and papaya in Cuba. Int J Syst Evol Microbiol 55, 2451–2463.[CrossRef] [Google Scholar]
  5. Bertaccini, A., Carraro, L., Davies, D., Laimer da Camara Machado, M., Martini, M., Paltrinieri, S. & Seemüller, E.(2000).Micropropagation of a collection of phytoplasma strains in periwinkle and other host plants. In Proceedings of the 13th International Congress of the International Organization for Mycoplasmology, Fukuoka, Japan, 14–19 July 2000, p. 101.
  6. Chiykowski, I. N. & Sinha, R. C.(1989). Differentiation of MLO disease by means of symptomatology and vector transmission. Zentralbl Bakteriol Hyg 20 (Suppl.), 280–287. [Google Scholar]
  7. Deng, S. & Hiruki, C.(1991). Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14, 53–61.[CrossRef] [Google Scholar]
  8. Doyle, J. J. & Doyle, J. L.(1990). Isolation of plant DNA from fresh tissue. Focus 12, 13–15. [Google Scholar]
  9. Gundersen, D. E., Lee, I.-M., Rehner, S. A., Davis, R. E. & Kingsbury, D. T.(1994). Phylogeny of mycoplasma-like organisms (phytoplasmas): a basis for their classification. J Bacteriol 176, 5244–5254. [Google Scholar]
  10. Heiner, C. R., Hunkapillar, K. L., Chen, S. M., Glass, J. I. & Chen, E. Y.(1998). Sequencing multimegabase-template DNA with Big Dye Terminator Chemistry. Genome Res 8, 557–561. [Google Scholar]
  11. IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma Taxonomy Group(2004).Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54, 1243–1255.[CrossRef] [Google Scholar]
  12. Khan, A. J., Botti, S., Al-Subhi, A. M., Gundersen-Rindal, D. E. & Bertaccini, A.(2002). Molecular identification of a new phytoplasma strain associated with alfalfa witches'-broom in Oman. Phytopathology 92, 1038–1047.[CrossRef] [Google Scholar]
  13. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors)(1992).International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  14. Lee, I.-M., Bertaccini, A., Vibio, M. & Gundersen, D. E.(1995). Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy. Phytopathology 85, 728–735.[CrossRef] [Google Scholar]
  15. Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E. & Bartoszyk, I. M.(1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48, 1153–1169.[CrossRef] [Google Scholar]
  16. Lee, I.-M., Bottner, K. D., Secor, G. & Rivera-Varas, V.(2006).Candidatus Phytoplasma americanum’, a phytoplasma associated with a potato purple top wilt disease complex. Int J Syst Evol Microbiol 56, 1593–1597.[CrossRef] [Google Scholar]
  17. McCoy, R. E., Caudwell, A., Chang, C. J., Chen, T. A., Chikowsky, I. N., Cousin, M. T., Dale de Leeuw, G. T. N., Golino, D. A., Hackett, K. J. & other authors(1989). Plant diseases associate with mycoplasma-like organisms. In The Mycoplasmas, vol. 5, pp. 545–560. Edited by R. F. Whitcomb & J. G. Tully. New York: Academic Press.
  18. Murray, R. G. E. & Stackebrandt, E.(1995). Taxonomic note: implementation of the provisional status Candidatus for incompletely described prokaryotes. Int J Syst Bacteriol 45, 186–187.[CrossRef] [Google Scholar]
  19. Schneider, B., Seemüller, E., Smart, C. D. & Kirkpatrick, B. C.(1995). Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology, vol. 2, pp. 369–380. Edited by S. Razin & J. G. Tully. San Diego: Academic Press.
  20. Seemüller, E., Garnier, M. & Schneider, B.(2002). Mycoplasma of plants and insects. In Molecular Biology and Pathology of Mycoplasmas, pp. 91–116. Edited by S. Razin & R. Hermann. London: Kluwer Academic/Plenum Publishers.
  21. Swofford, D. L.(2002).paup*: Phylogenetic analysis using parsimony (*and other methods), version 4. Sunderland, MA: Sinauer Associates.
  22. Valiunas, D., Staniulis, J. & Davis, R. E.(2006).Candidatus Phytoplasma fragariae’, a novel phytoplasma taxon discovered in yellows diseased strawberry, Fragaria×ananassa. Int J Syst Evol Microbiol 56, 277–281.[CrossRef] [Google Scholar]
  23. Verdin, E., Salar, P., Danet, J. L., Choueiri, E., Jreijiri, F., El Zammar, S., Gelie, B., Bové, J. M. & Garnier, M.(2003).Candidatus Phytoplasma phoenicium’ sp. nov., a novel phytoplasma associated with an emerging lethal disease of almond trees in Lebanon and Iran. Int J Syst Evol Microbiol 53, 833–838.[CrossRef] [Google Scholar]

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vol. , part 2, pp. 461 - 466

Details of reference phytoplasmas used in RFLP analysis. [PDF](12 KB)


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