A novel moderately halophilic, Gram-negative, curved-rod-shaped bacterium, designated strain AF-2004, was isolated from Bakhtegan Lake, a hypersaline lake with 17 % (w/v) total salt located in the southern region of Iran. Strain AF-2004 was a facultative anaerobe, motile by one polar flagellum, non-sporulating and oxidase- and catalase-positive. It grew at salinities of 1–17 % (w/v) NaCl, showing optimal growth at 5 % (w/v). Growth occurred at 10.0–45.0 °C and pH 5.0–9.5, with optimum growth at 32.0–35.0 °C and pH 8.0–8.5. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain AF-2004 is a member of the genus . Strain AF-2004 had C 7 (31.6 %), C 7 (22.1 %) and C (20.7 %) as the predominant fatty acids and Q-8 as the major respiratory lipoquinone. The DNA G+C content was 49.5 mol%, which is in the range of values for members of the genus . On the basis of differentiation from by phenotypic and chemotaxonomic characteristics, 16S rRNA sequence analysis and DNA–DNA relatedness, it is proposed that strain AF-2004 (=DSM 19052 =CIP 109598) should be placed in the genus as the type strain of a novel species, sp. nov.


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