A novel thermophilic, strictly anaerobic archaeon, designated strain Arc51, was isolated from a rock sample collected from a deep-sea hydrothermal field in Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean. Cells of the isolate were irregular cocci with single flagella and exhibited blue–green fluorescence at 436 nm. The optimum temperature, pH and NaCl concentration for growth were 70 °C, pH 6.5 and 3 % (w/v), respectively. Strain Arc51 could grow on thiosulfate or sulfite as an electron acceptor in the presence of hydrogen. This strain required acetate as a carbon source for its growth, suggesting that the reductive acetyl CoA pathway for CO fixation was incomplete. In addition, coenzyme M (2-mercaptoethanesulfonic acid), which is a known methyl carrier in methanogenesis, was also a requirement for growth of the strain. Analysis of the 16S rRNA gene sequence revealed that the isolate was similar to members of the genus , with sequence similarities of 93.6–97.2 %; the closest relative was . Phylogenetic analyses of the and genes, encoding the alpha and beta subunits of dissimilatory sulfite reductase and the alpha subunit of adenosine-5′-phosphosulfate reductase, respectively, produced results similar to those inferred from comparisons based on the 16S rRNA gene sequence. On the basis of phenotypic and phylogenetic data, strain Arc51 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Arc51 (=NBRC 100649=DSM 18877).


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