A novel aerobic, Gram-negative, rod-shaped, motile bacterium, designated strain 5516S-11T, was isolated from air samples collected in the Suwon region of the Republic of Korea. Analysis of the 16S rRNA gene sequence indicated that the organism belongs to the genus Massilia; the highest sequence similarity (97.2 %) was found with respect to Massilia aurea DSM 18055T. Cells of strain 5516S-11T contained ubiquinone Q-8 as the predominant isoprenoid quinone and possessed summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH; 35.2 %), C16 : 0 (30.6 %) and C18 : 1ω7c (11.7 %) as the major fatty acids. DNA–DNA hybridization revealed 32 % relatedness between strain 5516S-11T and M. aurea DSM 18055T. The G+C content of the DNA of strain 5516S-11T was 68.9 mol%. It is clear from the genotypic and phenotypic data presented that strain 5516S-11T represents a novel species of the genus Massilia, for which the name Massilia aerilata sp. nov. is proposed. The type strain is 5516S-11T (=KACC 12505T =DSM 19289T).
Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20, 406–416.[CrossRef][Google Scholar]
Gallego, V., Sánchez-Porro, C., García, M. T. & Ventosa, A.(2006).Massilia aurea sp. nov., isolated from drinking water. Int J Syst Evol Microbiol56, 2449–2453.[CrossRef][Google Scholar]
Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol46, 234–239.[CrossRef][Google Scholar]
Hiraishi, A.(1992). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol15, 210–213.[CrossRef][Google Scholar]
Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform5, 150–163.[CrossRef][Google Scholar]
La Scola, B., Birtles, R. J., Mallet, M. N. & Raoult, D.(1998).Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. J Clin Microbiol36, 2847–2852.
[Google Scholar]
Lindquist, D., Murrill, D., Burran, W. P., Winans, G., Janda, J. M. & Probert, W.(2003). Characteristics of Massilia timonae and Massilia timonae-like isolates from human patients, with an emended description of the species. J Clin Microbiol41, 192–196.[CrossRef][Google Scholar]
Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39, 159–167.[CrossRef][Google Scholar]
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Seldin, L. & Dubnau, D.(1985). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol35, 151–154.[CrossRef][Google Scholar]
Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res22, 4673–4680.[CrossRef][Google Scholar]
Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37, 463–464.[CrossRef][Google Scholar]
Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol173, 697–703.
[Google Scholar]