sp. nov., isolated from an air sample Free

Abstract

A novel aerobic, Gram-negative, rod-shaped, motile bacterium, designated strain 5516S-11, was isolated from air samples collected in the Suwon region of the Republic of Korea. Analysis of the 16S rRNA gene sequence indicated that the organism belongs to the genus ; the highest sequence similarity (97.2 %) was found with respect to DSM 18055. Cells of strain 5516S-11 contained ubiquinone Q-8 as the predominant isoprenoid quinone and possessed summed feature 3 (C 7/iso-C 2-OH; 35.2 %), C (30.6 %) and C 7 (11.7 %) as the major fatty acids. DNA–DNA hybridization revealed 32 % relatedness between strain 5516S-11 and DSM 18055. The G+C content of the DNA of strain 5516S-11 was 68.9 mol%. It is clear from the genotypic and phenotypic data presented that strain 5516S-11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5516S-11 (=KACC 12505 =DSM 19289).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65419-0
2008-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1422.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65419-0&mimeType=html&fmt=ahah

References

  1. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  2. Gallego, V., Sánchez-Porro, C., García, M. T. & Ventosa, A.(2006).Massilia aurea sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56, 2449–2453.[CrossRef] [Google Scholar]
  3. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  4. Hiraishi, A.(1992). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef] [Google Scholar]
  5. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  6. La Scola, B., Birtles, R. J., Mallet, M. N. & Raoult, D.(1998).Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. J Clin Microbiol 36, 2847–2852. [Google Scholar]
  7. Lindquist, D., Murrill, D., Burran, W. P., Winans, G., Janda, J. M. & Probert, W.(2003). Characteristics of Massilia timonae and Massilia timonae-like isolates from human patients, with an emended description of the species. J Clin Microbiol 41, 192–196.[CrossRef] [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  9. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  10. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  11. Seldin, L. & Dubnau, D.(1985). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef] [Google Scholar]
  12. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  13. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  14. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  15. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]
  16. Zhang, Y.-Q., Li, W.-J., Zhang, K.-Y., Tian, X.-P., Jiang, Y., Xu, L.-H., Jiang, C.-L. & Lai, R.(2006).Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. Int J Syst Evol Microbiol 56, 459–463.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65419-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65419-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed