Strains originally identified phenotypically as members of the species were subjected to a more detailed classification employing 16S rRNA gene sequence analysis, DNA–DNA hybridization, determination of the DNA base composition and phenotypic characterization. The quinone system, consisting of the predominant compound ubiquinone Q-8 and minor amounts of menaquinone MK-8, the major components of the polar lipid profile, as well as the polyamine pattern, with putrescine as the major compound, supported the assignment of the strains to the genus . Based on DNA–DNA relatedness, a specific 16S rRNA gene sequence type, absence of melibiose fermentation and a polar lipid profile lacking phosphatidylmonomethylethanolamine and two aminolipids, the strains were identified as members of a novel species for which the name Y sp. nov. is proposed. The type strain of sp. nov. is strain Y228 (=CCUG 52882=LMG 23763).


Article metrics loading...

Loading full text...

Full text loading...



  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J.(1996). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef] [Google Scholar]
  2. Bercovier, H. & Mollaret, H. H.(1984). Genus XIV. Yersinia. In Bergey's Manual of Systematic Bacteriology, Vol. 1, pp. 498–506. Edited by N. R. Krieg & J. G. Holt. Baltimore, MD: Williams and Wilkins.
  3. Bercovier, H., Mollaret, H. H., Alonso, J. M., Brault, J., Fanning, G. R., Steigerwaldt, A. G. & Brenner, D. J.(1980). Intra- and interspecies relatedness of Yersinia pestis by DNA hybridization and its relatedness to Yersinia pseudotuberculosis. Curr Microbiol 4, 225–229.[CrossRef] [Google Scholar]
  4. Brenner, D. J., Steigerwaldt, A. G., Falcao, D. P., Weaver, R. E. & Fanning, G. R.(1976). Characterisation of Yersinia entercolitica and Yersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions. Int J Syst Bacteriol 26, 180–194.[CrossRef] [Google Scholar]
  5. Busse, H.-J. & Auling, G.(1988). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef] [Google Scholar]
  6. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  7. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  8. Escara, J. F. & Hutton, J. R.(1980). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef] [Google Scholar]
  9. Fukushima, H., Gomyoda, M., Hashimoto, N., Takashima, I., Shubin, F. N., Isachikova, L. M., Paik, I. K. & Zheng, X. B.(1998). Putative origin of Yersinia pseudotuberculosis in western and eastern countries. A comparison of restriction endonuclease analysis of virulence plasmids. Zentralbl Bakteriol 288, 93–102.[CrossRef] [Google Scholar]
  10. Fukushima, H., Matsuda, Y., Seki, R., Tsubokura, M., Takeda, N., Shubin, F. N., Paik, I. K. & Zheng, X. B.(2001). Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains. J Clin Microbiol 39, 3541–3547.[CrossRef] [Google Scholar]
  11. Hamana, K.(1996). Distribution of diaminopropane and acetylspermidine in Enterobacteriaceae. Can J Microbiol 42, 107–114.[CrossRef] [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Ibrahim, A., Goebel, B. M., Liesack, W., Griffiths, M. & Stackebrandt, E.(1993). The phylogeny of the genus Yersinia based on 16S rRNA sequences. FEMS Microbiol Lett 114, 173–178.[CrossRef] [Google Scholar]
  14. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2006 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  15. Kim, W., Song, M. O., Song, W., Kim, K. J., Chung, S. I., Choi, C. S. & Park, Y. H.(2003). Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis. Antonie van Leeuwenhoek 83, 125–133.[CrossRef] [Google Scholar]
  16. Martin, K., Schumann, P., Rainey, F. A., Schuetze, B. & Groth, I.(1997).Janibacter limosus gen. nov., sp. nov., a new actinomycete with meso-diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47, 529–534.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W.(1989). Precise measurement of the G+C content of deoxyribonucleic acids by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Nagano, T., Kiyohara, T., Suzuki, K., Tsubokura, M. & Otsuki, K.(1997). Identification of pathogenic strains within serogroups of Yersinia pseudotuberculosis and the presence of non-pathogenic strains isolated from animals and the environment. J Vet Med Sci 59, 153–158.[CrossRef] [Google Scholar]
  19. Neubauer, H., Meyer, H., Prior, J., Aleksic, S., Hensel, A. & Splettstösser, W.(2000a). A combination of different polymerase chain reaction (PCR) assays for the presumptive identification of Yersinia pestis. J Vet Med B Infect Dis Vet Public Health 47, 573–580.[CrossRef] [Google Scholar]
  20. Neubauer, H., Hensel, A., Aleksic, S., Finke, E.-J. & Meyer, H.(2000b).Yersinia enterocolitica 16S rRNA gene types belong to the same genospecies but form three different homology clusters. Int J Med Microbiol 290, 61–64.[CrossRef] [Google Scholar]
  21. Neubauer, H., Sprague, L. D., Hensel, A., Aleksic, S. & Meyer, H.(2000c). Specific detection of plasmid bearing Yersinia isolates by PCR. Clin Lab 46, 583–587. [Google Scholar]
  22. Neubauer, H., Molitor, M., Rahalison, L., Aleksic, S., Backes, H., Chanteau, S. & Meyer, H.(2000d). A semi-automated system for identification of Yersinia species within the genus Yersinia. Clin Lab 46, 561–567. [Google Scholar]
  23. Sprague, L. D. & Neubauer, H.(2005).Yersinia aleksiciae sp. nov. Int J Syst Evol Microbiol 55, 831–835.[CrossRef] [Google Scholar]
  24. Stolz, A., Busse, H.-J. & Kämpfer, P.(2007).Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef] [Google Scholar]
  25. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  26. Tindall, B. J.(1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef] [Google Scholar]
  27. Tindall, B. J.(1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  28. Tsubokura, M. & Aleksic, S.(1995). A simplified antigenetic scheme for serotyping of Yersinia pseudotuberculosis: phenotypic characterisation of reference strains and preparation of O and H factor sera. Contrib Microbiol Immunol 13, 99–105. [Google Scholar]
  29. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K.(1992). Distribution of quinone systems in microorganisms: Gram-negative eubacteria. Bull JFCC 8, 136–171. [Google Scholar]

Data & Media loading...


vol. , part 4, pp. 952 - 958

Results of DNA-DNA hybridization (%) between strains of and sp. nov. [ PDF]


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error