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Abstract

We have carried out a polyphasic taxonomic study of strain 11S, a halophilic, Gram-negative bacterium that is able to respire on nitrate and nitrite in anaerobiosis. Strain 11S was isolated from a solar saltern in Cahuil, a region next to Pichilemu (Chile). It grows at NaCl concentrations within the range of 3–20 % w/v (optimum 5–7.5 %), temperatures from 4 to 45 °C (optimum 20–32 °C) and within a pH range of 5–10 (optimum pH 7–9). Its 16S rRNA gene sequence indicates that it belongs to the genus in the class . Its closest relatives are , , and , with the type strains of which our strain showed maximum 16S rRNA gene sequence similarity values of 97.1–98.1 %. Its G+C content is 65.3 mol%. DNA–DNA hybridization studies showed 54.2 % relatedness between strain 11S and DSM 15356 and 47.2 % relatedness between strain 11S and CECT 5995. Lower DNA–DNA hybridization percentages were obtained against the type strains of other related species. Its major fatty acids are C 3-OH (5.56 %), iso-C 2-OH/C 7 (22.30 %), C (27.80 %) and C 7 (29.92 %). The proposed name for the novel species is sp. nov., with strain 11S (=CECT 7281 =LMG 24185) being the type strain.

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2008-04-01
2019-12-14
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vol. , part 4, pp. 872 - 876

Dendrogram based on 107 phenotypic characteristics.

Phylogenetic tree, based on 16S rRNA gene sequences, showing the position of strain 11S with respect to other members of the family .

[PDF file of Supplementary Figs S1 and S2](22 KB)



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