The taxonomic status of six strains received as was established using a polyphasic approach. The organisms had phenotypic and morphological properties consistent with their classification in the genus . Almost complete 16S rRNA gene sequences of the strains were determined and aligned with corresponding sequences of representatives of the genus and phylogenetic trees were inferred using four tree-making algorithms. All of the strains fell within the 16S rRNA gene clade and four of them were considered to merit species status using a combination of genotypic and phenotypic data. These organisms were designated sp. nov. (type strain ATCC 53653=DSM 41914), sp. nov. (type strain NRRL B-1346=DSM 40771), sp. nov. (type strain NRRL 5491=ATCC 29253) and sp. nov. (type strain ISP 5276=DSM 40276). DNA–DNA relatedness and phenotypic data showed that the two remaining strains, ‘ subsp. ’ NRRL 3664 and subsp. NRRL 3111 belong to the same species as the type strain of subsp. . This taxon is designated sp. nov., comb. nov. (type strain ATCC 15191=DSM 40830) following the proposal that subsp. is a heterotypic synonym of .


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  1. Anderson, A. S. & Wellington, E. M. H.(2001). The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51, 797–814.[CrossRef] [Google Scholar]
  2. Atalan, E., Manfio, G. P., Ward, A. C., Kroppenstedt, R. M. & Goodfellow, M.(2000). Biosystematic studies on novel streptomycetes from soil. Antonie Van Leeuwenhoek 77, 337–353.[CrossRef] [Google Scholar]
  3. Cercós, A. P.(1954).Streptomyces rutgersensis var. castelarense n. var. Nuevas propiedades de la canfomicina. Rev Invest Agric 8, 263–283 (in Spanish). [Google Scholar]
  4. Chamberlain, K. & Crawford, D. L.(1999).In vitro and in vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopicus strains YCED9 and WYE 53. J Ind Microbiol Biotechnol 23, 641–646.[CrossRef] [Google Scholar]
  5. DSMZ(1998).Catalogue of Strains. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen.
  6. Duangmal, K., Ward, A. C. & Goodfellow, M.(2005). Selective isolation of members of the Streptomyces violaceoruber clade from soil. FEMS Microbiol Lett 245, 321–327.[CrossRef] [Google Scholar]
  7. Ettlinger, L., Corbaz, R. & Hütter, R.(1958). Zur Systematik der Actinomyceten. 4. Eine Arteinteilung der Gattung Streptomyces Waksman & Henrici. Arch Mikrobiol 31, 326–358.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1993).phylip (phylogeny inference package) version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Ferguson, E. V., Ward, A. C., Sanglier, J. J. & Goodfellow, M.(1997). Evaluation of Streptomyces species-groups by pyrolysis mass spectrometry. Zentralbl Bakteriol 285, 169–181.[CrossRef] [Google Scholar]
  12. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  13. Gonzalez, J. M. & Saiz-Jimenez, C.(2005). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9, 75–79.[CrossRef] [Google Scholar]
  14. Goodfellow, M., Ferguson, E. V. & Sanglier, J. J.(1992). Numerical classification and identification of Streptomyces species: a review. Gene 115, 225–233.[CrossRef] [Google Scholar]
  15. Goodfellow, M., Manfio, G. P. & Chun, J.(1997). Towards a practical species concept for cultivable bacteria. In Species, The Units of Biodiversity, pp. 25–59. Edited by M. F. Claridge, H. A. Dawah & M. R. Wilson. London: Chapman & Hall.
  16. Goodfellow, M., Kumar, Y., Labeda, D. P. & Sembiring, L.(2007). The Streptomyces violaceusniger clade: a home for streptomycetes with rugose ornamented spores. Antonie Van Leeuwenhoek 92, 173–199.[CrossRef] [Google Scholar]
  17. Johnson, L. E. & Dietz, A.(1971). Scopafungin, a crystalline antibiotic produced by Streptomyces hygroscopicus var. enhygrus var. nova. Appl Microbiol 22, 303–308. [Google Scholar]
  18. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  19. Jurado, V., Laiz, L., Gonzalez, J. M., Hernandez-Marine, M., Valens, M. & Saiz-Jimenez, C.(2005).Phyllobacterium catacumbae sp. nov., a member of the order ‘Rhizobiales’ isolated from Roman catacombs. Int J Syst Evol Microbiol 55, 1487–1490.[CrossRef] [Google Scholar]
  20. Kämpfer, P., Kroppenstedt, R. M. & Dott, W.(1991). A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137, 1831–1891.[CrossRef] [Google Scholar]
  21. Kino, T., Hatanaka, H., Hashimoto, M., Nishiyama, M., Goto, T., Okuhara, M., Kohsaka, M., Aoki, H. & Imanaka, H.(1987). FK-506, a novel immunosuppressant isolated from a Streptomyces. I. Fermentation, isolation, and physico-chemical and biological characteristics. J Antibiot (Tokyo) 40, 1249–1255.[CrossRef] [Google Scholar]
  22. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  23. Kumar, Y., Aiemsum-ang, P., Ward, A. C. & Goodfellow, M.(2007). Diversity and geographical distribution of members of the Streptomyces violaceusniger 16S rRNA gene clade detected by clade specific PCR primers. FEMS Microbiol Ecol 62, 54–63.[CrossRef] [Google Scholar]
  24. Labeda, D. P.(1993). DNA relatedness among strains of the Streptomyces lavendulae phenotypic cluster group. Int J Syst Bacteriol 43, 822–825.[CrossRef] [Google Scholar]
  25. Labeda, D. P.(1998). DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48, 829–832.[CrossRef] [Google Scholar]
  26. Labeda, D. P. & Lyons, A. J.(1991). The Streptomyces violaceusniger cluster is heterogeneous in DNA relatedness among strains: emendation of the descriptions of Streptomyces violaceusniger and Streptomyces hygroscopicus. Int J Syst Bacteriol 41, 398–401.[CrossRef] [Google Scholar]
  27. Labeda, D. P. & Lyons, A. J.(1992). DNA relatedness among strains of the sweet potato pathogen Streptomyces ipomoea (Person and Martin 1940) Waksman and Henrici 1948. Appl Environ Microbiol 58, 532–535. [Google Scholar]
  28. Lam, K. S., Hesler, G. A., Mattei, J. M., Mamber, S. W., Forenza, S. & Tomita, K.(1990). Himastatin, a new antitumour antibiotic from Streptomyces hygroscopicus. I. Taxonomy of producing organism, fermentation and biological activity. J Antibiot (Tokyo) 43, 956–990.[CrossRef] [Google Scholar]
  29. Lanoot, B., Vancanneyt, M., Dawyndt, P., Cnockaert, M., Zhang, J., Huang, Y., Liu, Z. & Swings, J.(2004). Box-PCR fingerprinting as a powerful tool to reveal synonymous names of the genus Streptomyces. Emended descriptions are proposed for the species Streptomyces cinereorectus, S. fradiae, S. tricolor, S. colombiensis, S. filamentosus, S. vinaceus and S. phaeopurpureus. Syst Appl Microbiol 27, 84–92.[CrossRef] [Google Scholar]
  30. Lanoot, B., Vancanneyt, M., Hoste, B., Vandameulebroecke, K., Cnockaert, M. C., Dawyndt, P., Liu, Z., Huang, Y. & Swings, J.(2005). Grouping streptomycetes using 16S-ITS RFLP fingerprinting. Res Microbiol 156, 755–762.[CrossRef] [Google Scholar]
  31. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors)(1992).International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  32. Liu, Z., Shi, Y., Zhang, Y., Zhou, Z., Lu, Z., Li, W., Huang, Y., Rodriguez, C. & Goodfellow, M.(2005). Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of ‘Microstreptospora cinerea’ to the genus Streptomyces as Streptomyces yanii sp. nov. Int J Syst Evol Microbiol 55, 1605–1610.[CrossRef] [Google Scholar]
  33. Manchester, L., Pot, B., Kersters, K. & Goodfellow, M.(1990). Classification of Streptomyces and Streptoverticillium species by numerical analysis of electrophoretic protein patterns. Syst Appl Microbiol 13, 333–337.[CrossRef] [Google Scholar]
  34. Manfio, G. P., Zakrzewska-Czerwinska, J., Atalan, E. & Goodfellow, M.(1995). Towards minimal standards for the description of Streptomyces species. Biotekhnologia 7–8, 242–253. [Google Scholar]
  35. Mordarski, M., Goodfellow, M., Williams, S. T. & Sneath, P. H. A.(1986). Evaluation of species-groups in the genus Streptomyces. In Biological, Biochemical, and Biomedical Aspects of Actinomycetes, pp. 517–525. Edited by G. Szabó, S. Biró & M. Goodfellow. Budapest: Akadémiai Kiadó.
  36. Nam, S.-W., Kim, W., Chun, J. & Goodfellow, M.(2004).Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54, 1209–1212.[CrossRef] [Google Scholar]
  37. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  38. Rossi Doria, T.(1891). Su di alcune specie di “Streptothrix” trovate nell’aria studate in rapporto a quelle giá note a specialmente all’ “Actinomyces”. Ann Int Igiene Sper Univ Roma 1, 399–438 (in Italian). [Google Scholar]
  39. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–428. [Google Scholar]
  40. Sembiring, L., Ward, A. C. & Goodfellow, M.(2000). Selective isolation and characterisation of members of the Streptomyces violaceusniger clade associated with the roots of Paraserianthes falcataria. Antonie van Leeuwenhoek 78, 353–366.[CrossRef] [Google Scholar]
  41. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  42. Shirling, E. B. & Gottlieb, D.(1968). Cooperative description of type cultures of Streptomyces. II. Species description from first study. Int J Syst Bacteriol 18, 69–189.[CrossRef] [Google Scholar]
  43. Sveshnikova, M. A.(1957). In Problems of Classification of Actinomycetes – Antagonists, pp. 1–398. Edited by G. F. Gauze, T. P. Preobrazhenskaya, E. S. Kudrina, N. O. Blinov, I. D. Ryabova & M. A. Sveshnikova. Moscow: Government Publishing House of Medical Literature, Mediz.
  44. Tripathi, C. K. M., Praveen, V., Singh, V. & Bihari, V.(2004). Production of antibacterial and antifungal metabolites by Streptomyces violaceusniger and media optimization studies for the maximum metabolite production. Med Chem Res 13, 790–799.[CrossRef] [Google Scholar]
  45. Vezina, C., Kudelski, A. & Sehgal, S.(1975). Rapamycin (AY-22, 989), a new antifungal antibiotic. I. Taxonomy of the producing streptomycete and isolation of the active principle. J Antibiot (Tokyo) 28, 721–726.[CrossRef] [Google Scholar]
  46. Waksman, S. A. & Curtis, R. E.(1916). The Actinomyces of the soil. Soil Sci 1, 99–134.[CrossRef] [Google Scholar]
  47. Waksman, S. A. & Henrici, A. T.(1948). Family III. Streptomycetaceae Waksman and Henrici. In Bergey's Manual of Determinative Bacteriology, 6th edn, pp. 929–980. Edited by R. S. Breed, R. G. E. Murray & A. P. Hitchens. Baltimore: Williams & Wilkins.
  48. Wayne, L. B., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  49. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  50. Williams, S. T., Goodfellow, M. & Alderson, G.(1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  51. Xu, C., Wang, L., Cui, Q., Huang, Y., Liu, Z., Zhang, G. & Goodfellow, M.(2006). Novel neutrotolerant acidophilic Streptomyces species isolated from a soil in China: Streptomyces guandensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov. Int J Syst Evol Microbiol 56, 1109–1115.[CrossRef] [Google Scholar]
  52. Zbinovsky, V., Hausmann, W. K., Wetzel, E. R., Borders, D. B. & Patterson, E. L.(1968). Isolation and characterisation of antibiotic LL-AC 541. Appl Microbiol 16, 614–616. [Google Scholar]

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