Two Gram-negative, rod-shaped, non-spore-forming bacteria, PR17 and DSM 7216, isolated from the potato rhizosphere and an industrial environment, respectively, were studied for their taxonomic allocation. By (16S rRNA) gene sequencing, these strains were shown to belong to the , most closely related to (98.4 and 99.3 % similarity to the type strain, respectively). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, -homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine and the -specific unidentified aminolipid AL2; major fatty acids C 7 and C cyclo 8) supported the genus affiliation. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from all hitherto-described species. Hence, both isolates represent novel species of the genus , for which the names sp. nov. (type strain PR17 =CCUG 55411 =CCM 7493 =DSM 19824) and sp. nov. (type strain DSM 7216 =CCUG 55412 =CCM 7492) are proposed.


Article metrics loading...

Loading full text...

Full text loading...



  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J.(1996). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef] [Google Scholar]
  2. Busse, H.-J. & Auling, G.(1988). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef] [Google Scholar]
  3. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  4. Holmes, B., Popoff, M., Kiredjian, M. & Kersters, K.(1988).Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as group Vd. Int J Syst Bacteriol 38, 406–416.[CrossRef] [Google Scholar]
  5. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  6. Kämpfer, P., Steiof, M. & Dott, W.(1991). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef] [Google Scholar]
  7. Kämpfer, P., Buczolits, S., Albrecht, A., Busse, H.-J. & Stackebrandt, E.(2003). Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53, 893–896.[CrossRef] [Google Scholar]
  8. Kämpfer, P., Rosselló-Mora, R., Scholz, H., Welinder-Olsen, C. & Busse, H.-J.(2006). Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp. nov. and Pseudochrobactrum saccharolyticum sp. nov. Int J Syst Evol Microbiol 56, 1823–1829.[CrossRef] [Google Scholar]
  9. Kämpfer, P., Scholz, H., Huber, B., Thummes, K., Busse, H.-J., Maas, E. W. & Falsen, E.(2007a). Description of Pseudochrobactrum kiredjianiae sp. nov. Int J Syst Evol Microbiol 57, 755–760.[CrossRef] [Google Scholar]
  10. Kämpfer, P., Scholz, H. C., Falsen, E. & Busse, H.-J.(2007b).Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 57, 2513–2518.[CrossRef] [Google Scholar]
  11. Kreimer, S.(1992).Untersuchungen zum Stoffwechsel von Thiophen-2-carboxylat in Agrobacterium tumefaciens und Ochrobactrum anthropi und Reindarstellung einer CoA-Esterase. Doctoral thesis, University Göttingen, Germany (in German).
  12. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  13. Lebuhn, M., Achouak, W., Schloter, M., Berge, O., Meier, H., Barakat, M., Hartmann, A. & Heulin, T.(2000). Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov. Int J Syst Evol Microbiol 50, 2207–2223.[CrossRef] [Google Scholar]
  14. Lechner, U., Baumbach, R., Becker, D., Kitunen, V., Auling, G. & Salkinoja-Salonen, M.(1995). Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description. Biodegradation 6, 83–92.[CrossRef] [Google Scholar]
  15. Scholz, H. C., Tomaso, H., Al Dahouk, S., Witte, A., Schloter, M., Kämpfer, P., Falsen, E. & Neubauer, H.(2006). Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257, 7–16.[CrossRef] [Google Scholar]
  16. Stolz, A., Busse, H.-J. & Kämpfer, P.(2007).Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef] [Google Scholar]
  17. Teyssier, C., Marchandin, H., Jean-Pierre, H., Masnou, A., Dusart, G. & Jumas-Bilak, E.(2007).Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from man. Int J Syst Evol Microbiol 57, 1007–1013.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  19. Tindall, B. J.(1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef] [Google Scholar]
  20. Tindall, B. J.(1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  21. Tripathi, A. K., Verma, S. C., Chowdhury, S. P., Lebuhn, M., Gattinger, A. & Schloter, M.(2006).Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. Int J Syst Evol Microbiol 56, 1677–1680.[CrossRef] [Google Scholar]
  22. Trujillo, M. E., Willems, A., Abril, A., Planchuelo, A. M., Rivas, R., Ludeña, D., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2005). Nodulation of Lupinus by strains of the new species Ochrobactrum lupini sp. nov. Appl Environ Microbiol 71, 1318–1327.[CrossRef] [Google Scholar]
  23. Velasco, J., Romero, C., López-Goñi, I., Leiva, J., Díaz, R. & Moriyón, I.(1998). Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol 48, 759–768.[CrossRef] [Google Scholar]
  24. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K.(1992). Distribution of quinone systems in microorganisms: gram-negative Eubacteria. Bull Jpn Fed Cult Coll 8, 136–171. [Google Scholar]
  25. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R.(1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef] [Google Scholar]
  26. Zurdo-Piñeiro, J. L., Rivas, R., Trujillo, M.E., Vizcaíno, N., Carrasco, J. A., Chamber, M., Palomares, A., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2007).Ochrobactrum cytisi sp. nov. isolated from nodules of Cytisus scoparius in Spain. Int J Syst Evol Microbiol 57, 784–788.[CrossRef] [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error