Five psychrophilic bacterial strains were isolated from soil samples collected above the treeline of alpine environments. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these organisms represent four novel species of the genus ; levels of sequence similarity to the type strains of recognized species were in the range 89.3–94.7 %. Strains PO-04-20-132, PO-04-20-144, PO-04-19-125, ME-04-01-32 and ME-04-04-52 grew aerobically, with optimum growth at 10 °C and at pH 6–9. The major respiratory menaquinone was MK-8. The fatty acid profiles of strains PO-04-20-132, PO-04-20-144, PO-04-19-125 and ME-04-01-32 were dominated by 16 : 17, 17 : 0 iso and 15 : 16, whereas 16 : 17, 17 : 0 cyclo and 16 : 0 predominated in strain ME-04-04-52. The DNA G+C contents of strains PO-04-20-132, PO-04-19-125, ME-04-01-32 and ME-04-04-52 were 63.2, 63.1, 65.9 and 62.6 mol%, respectively. Strains PO-04-20-132, PO-04-19-125, ME-04-01-32 and ME-04-04-52 had gamma radiation D (dose required to reduce the bacterial population by 10-fold) values of ≤4 kGy. These four strains showed sensitivity to UV radiation and extended desiccation as compared with . On the basis of the phylogenetic analyses, and chemotaxonomic and phenotypic data, it is proposed that strains PO-04-20-132 (=LMG 24019=NRRL B-41950; sp. nov.), PO-04-19-125 (=LMG 24282=NRRL B-41949; sp. nov.), ME-04-01-32 (=LMG 24022=NRRL B-41947; sp. nov.) and ME-04-04-52 (=LMG 24283=NRRL B-41948; sp. nov.) represent the type strains of four novel species of the genus .


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Two-dimensional thin-layer chromatography of the total polar lipids of the four new isolates and . [PDF](113 KB)


Graphs showing the effect of gamma radiation, UV radiation and desiccation on the survival of the four new isolates. [PDF](90 KB)


Fatty acid compositions of the four new isolates and recognized species. [PDF](58 KB)

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