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Abstract

A novel sulfate-reducing bacterium (strain JS1) was isolated from lake Pangong, a salt-water lake situated in the Himalayas of Leh District, Jammu and Kashmir, India. Cells of strain JS1 are Gram-negative, vibriod, motile by means of single polar flagellum, contain desulfoviridin, are catalase-positive and can hydrolyse starch. Strain JS1 grew optimally at pH 8.5–9.0 and at temperatures ranging from 10 to 50 °C (optimum 28–30 °C). Chemo-organoheterotrophy was the only growth mode of strain JS1 and was possible on a wide range of organic compounds. Sulfate alone but not sulfite, thiosulfate, elemental sulfur, nitrite or nitrate was used as electron acceptor. It has no requirement for NaCl but can tolerate up to 2 % NaCl (w/v). -Aminobenzoate is a required growth factor. The 16S rRNA gene sequence of the bacterium demonstrated similarity between strain JS1 and the members of the genus . The position of strain JS1 within the phylogenetic tree indicated that it clustered closely with DSM 10141 (89.85 % sequence similarity). On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JS1 be classified as a novel species of the genus of the family , with the species name sp. nov. The type strain of the species is JS1 (=JCM 14597=KCTC 5573=DSM 19430).

Keyword(s): PABA, p-aminobenzoate
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2008-04-01
2019-10-23
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References

  1. Abildgaard, L., Nielsen, M. B., Kjeldsen, K. U. & Ingvorsen, K. ( 2006; ). Desulfovibrio alkalitolerans sp. nov., a novel alkalitolerant, sulphate-reducing bacterium isolated from district heating water. Int J Syst Evol Microbiol 56, 1019–1024.[CrossRef]
    [Google Scholar]
  2. Bale, S. J., Goodman, K., Rochelle, P. A., Marchesi, J. R., Fry, J. R., Weightman, A. J. & Parkes, R. J. ( 1997; ). Desulfovibrio profundus sp. nov., a novel barophilic sulfate-reducing bacterium from deep sediment layers in the Japan sea. Int J Syst Bacteriol 47, 515–521.[CrossRef]
    [Google Scholar]
  3. Baron, E. J. ( 1997; ). Bilophila wadsworthia: a unique Gram-negative anaerobic rod. Anaerobe 3, 83–86.[CrossRef]
    [Google Scholar]
  4. Basso, O., Caumette, P. & Magot, M. ( 2005; ). Desulfovibrio putealis sp. nov., a novel sulfate-reducing bacterium isolated from a deep subsurface aquifer. Int J Syst Evol Microbiol 55, 101–104.[CrossRef]
    [Google Scholar]
  5. Cappuccino, J. G. & Sherman, N. ( 1998; ). Microbiology: a Laboratory Manual, 5th edn. Menlo Park, CA: Benjamin Cummings.
  6. Eichler, B. & Pfennig, N. ( 2001; ). A new green sulfur bacterium from a freshwater pond. In Green Photosynthetic Bacteria, pp. 233–235. Edited by J. Olson, E. Stackebrandt & H. G. Trüper. New York: Plenum.
  7. Felsenstein, J. ( 1989; ). phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Imhoff, J. F., Süling, J. & Petri, R. ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef]
    [Google Scholar]
  9. Kuever, J., Rainey, F. A. & Widdel, F. ( 2005; ). Family I. Desulfovibrionaceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria), p. 926. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  10. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  11. McOrist, S., Gebhart, C. J., Boid, R. & Barns, S. M. ( 1995; ). Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy. Int J Syst Bacteriol 45, 820–825.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Pikovskaya, R. E. ( 1948; ). Mobilization of phosphorus in soil in connection with the vital activity of some of the microbial species. Mikrobiologiia 17, 362–370 (in Russian).
    [Google Scholar]
  14. Postgate, J. R. ( 1984; ). The Sulphate-Reducing Bacteria. 2nd edn. Cambridge: Cambridge University Press.
  15. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch. & Ramana, Ch. V. ( 2007; ). Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57, 228–232.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. van der Maarel, M. J. E. C., van Bergeijk, S., van Werkhoven, A. F., Laverman, A. M., Meijer, W. G., Stam, W. T. & Hansen, T. A. ( 1996; ). Cleavage of dimethylsulfoniopropionate and reduction of acrylate by Desulfovibrio acrylicus sp. nov. Arch Microbiol 166, 109–115.[CrossRef]
    [Google Scholar]
  18. Vandieken, V., Knoblauch, C. & Jørgensen, B. B. ( 2006; ). Desulfovibrio frigidus sp. nov. and Desulfovibrio ferrireducens sp. nov., psychrotolerant bacteria isolated from Arctic fjord sediments (Svalbard) with the ability to reduce Fe(III). Int J Syst Evol Microbiol 56, 681–685.[CrossRef]
    [Google Scholar]
  19. Voordouw, G. ( 1995; ). The genus Desulfovibrio: the centennial. Appl Environ Microbiol 61, 2813–2819.
    [Google Scholar]
  20. Warren, Y. A., Citron, D. M., Merriam, C. V. & Goldstein, E. J. C. ( 2005; ). Biochemical differentiation and comparison of Desulfovibrio species and other phenotypically similar genera. J Clin Microbiol 43, 4041–4045.[CrossRef]
    [Google Scholar]
  21. Widdel, F. & Bak, F. ( 1992; ). Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes, 2nd edn, pp. 3353–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
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vol. , part 4, pp. 821–825

TEM showing the monopolar flagellum of strain JS1 . [PDF](29 KB)



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