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A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1T) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1T grew in the presence of 2 % NaCl at 30 °C and was characterized chemotaxonomically by having C16 : 0 as the major fatty acid followed by C17 : 0 cyclo and C18 : 1 ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Esp-1T formed a cluster together with Pseudomonas knackmussii DSM 6978T (98.9 % sequence similarity), Pseudomonas delhiensis MTCC 7601T (98.5 %), Pseudomonas nitroreducens DSM 14399T (98.5 %), Pseudomonas citronellolis DSM 50332T (98.7 %), Pseudomonas multiresinivorans ATCC 700690T (98.9 %) and Pseudomonas jinjuensis DSM 16612T (97.8 %). DNA–DNA hybridization values of strain Esp-1T with P. knackmussii DSM 6978T, P. delhiensis MTCC 7601T, P. jinjuensis DSM 16612T, P. citronellolis DSM 50332T, P. multiresinivorans ATCC 700690T and P. nitroreducens DSM 14399T were 32.9, 30.2, 20.6, 23.4, 23.4 and 20.0 %, respectively. Low levels of DNA–DNA hybridization and phenotypic and chemotaxonomic results are sufficient to delineate strain Esp-1T from other closely related species of Pseudomonas. Phenotypic and chemotaxonomic data confirm that strain Esp-1T represents a novel species, for which the name Pseudomonas panipatensis sp. nov. is proposed. The type strain of Pseudomonas panipatensis sp. nov. is Esp-1T (=MTCC 8990T=CCM 7469T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 6, pp. 1339 - 1345
Supplementary Table S1. DNADNA relatedness (%) of strain Esp-1 Twith phylogeetically related Pseudomonas strains [PDF](23 KB)