A novel halophilic archaeon, strain BZ256, was isolated from Zodletone Spring, a sulfide- and sulfur-rich spring in south-western Oklahoma, USA. Cells were non-motile, non-flagellated cocci that divided along two axes, resulting in the formation of sarcina-like clusters. Strain BZ256 grew at salt concentrations ranging from 1.3 to 4.3 M NaCl, with optimum growth at approximately 3.4 M, and required at least 1 mM Mg for growth. The pH range for growth was 5.0 to at least 8.5, and the temperature range for growth was 25–45 °C. The two diether phospholipids that are typical of members of the order , namely phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, were present in strain BZ256, as were two glycolipids chromatographically identical to S-DGD-1 and DGD-1. The 16S rRNA gene sequence of strain BZ256 showed 96.8 % similarity to that of the type strain of , the closest recognized species within the order . The DNA G+C content of strain BZ256 was 65.4 mol%. Microscopic, physiological, biochemical and phylogenetic comparisons between strain BZ256 and recognized genera of extremely halophilic archaea suggest that this strain represents a member of a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is BZ256 (=KCTC 4017 =JCM 14848).


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vol. , part 4, pp. 856 - 860

Analysis of the lipid composition of strain BZ256 using TLC. (a) One-dimensional TLC stained with orcinol reagent. Lanes: 1, R1; 2, strain; 3, strain; 4, strain; 5, PR 3 ; 6 and 7, DX253 ; 8, strain BZ256 . (b) Two-dimensional TLC stained for phospholipids, showing the presence of phosphatidylglycerol (PG) and phosphatidylglycerol phosphate methyl ester (Me-PGP) in strain BZ256 and the absence of phosphatidylglycerol sulfate. (c) Two-dimensional TLC stained with α-naphthol-sulfuric acid, showing the presence of two glycolipids in strain BZ256 .


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