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Two Gram-negative, rod-shaped, gliding bacterial strains, designated strain LL04 11.1.1T and strain A37.1T, were isolated from a diseased sole (Solea senegalensis) and from seawater from a holding tank for turbot (Psetta maxima), respectively. The strains grew on solid media as bright yellow colonies with uneven edges; the colonies did not adhere to the agar. The bacteria were able to grow at temperatures in the range 14 to 38 °C and from pH 6.0 to pH 8.0. The DNA G+C contents of strains LL04 11.1.1T and A37.1T were 32.1 and 32.7 mol%, respectively. Analysis of the 16S rRNA gene sequences indicated that strains LL04 11.1.1T and A37.1T were members of the genus Tenacibaculum in the family Flavobacteriaceae. The sequence similarities of the two isolates with respect to the type strains of recognized members of the genus ranged from 94.2 to 99.4 %. DNA–DNA hybridization studies revealed that the strains studied represent two distinct novel species of the genus Tenacibaculum, for which the names Tenacibaculum discolor sp. nov. [type strain LL04 11.1.1T (=NCIMB 14278T=DSM 18842T)] and Tenacibaculum gallaicum sp. nov. [type strain A37.1T (=NCIMB 14147T=DSM 18841T)] are proposed.
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