1887

Abstract

Two Gram-negative, rod-shaped, gliding bacterial strains, designated strain LL04 11.1.1 and strain A37.1, were isolated from a diseased sole () and from seawater from a holding tank for turbot (), respectively. The strains grew on solid media as bright yellow colonies with uneven edges; the colonies did not adhere to the agar. The bacteria were able to grow at temperatures in the range 14 to 38 °C and from pH 6.0 to pH 8.0. The DNA G+C contents of strains LL04 11.1.1 and A37.1 were 32.1 and 32.7 mol%, respectively. Analysis of the 16S rRNA gene sequences indicated that strains LL04 11.1.1 and A37.1 were members of the genus in the family . The sequence similarities of the two isolates with respect to the type strains of recognized members of the genus ranged from 94.2 to 99.4 %. DNA–DNA hybridization studies revealed that the strains studied represent two distinct novel species of the genus , for which the names sp. nov. [type strain LL04 11.1.1 (=NCIMB 14278=DSM 18842)] and sp. nov. [type strain A37.1 (=NCIMB 14147=DSM 18841)] are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65397-0
2008-01-01
2024-10-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/21.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65397-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  3. Buck, J. D.(1982). Non-staining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  5. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C.(2006).Tenacibaculum litoreum sp. nov, isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef] [Google Scholar]
  6. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  8. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  9. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N.(2004).Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef] [Google Scholar]
  10. Hansen, G. H., Bergh, O., Michaelsen, J. & Knappskog, D.(1992).Flexibacter ovolyticus sp. nov. a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42, 451–458.[CrossRef] [Google Scholar]
  11. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  12. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H.(2006).Tenacibaculum aestuarii sp. nov. isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef] [Google Scholar]
  13. Kimura, M.(1980). A simple model for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. Pazos, F., Santos, Y., Macías, A. R., Núñez, S. & Toranzo, A. E.(1996). Evaluation of media for the successful culture of Flexibacter maritimus. J Fish Dis 19, 193–197.[CrossRef] [Google Scholar]
  16. Piñeiro-Vidal, M., Centeno-Sestelo, G., Riaza, A. & Santos, Y.(2007). Isolation of pathogenic Tenacibaculum maritimum-related organisms from diseased turbot and sole cultured in the Northwest of Spain. Bull Eur Assoc Fish Pathol 27, 29–35. [Google Scholar]
  17. Reichenbach, H.(1989). The order Cytophagales Leadbetter 1974, 99AL. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2073. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. C. Holt. Baltimore: Williams & Wilkins.
  18. Reichenbach, H.(1992a). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  19. Reichenbach, H.(1992b).Flavobacteriaceae fam. nov. In Validation of the Publication of the New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42, 327–329.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sheu, S.-Y., Lin, K.-Y., Chou, J.-H., Chang, P.-S., Arun, A. B., Young, C.-C. & Chen, W.-M.(2007).Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef] [Google Scholar]
  22. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S.(2001). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef] [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  24. Wakabayashi, H., Hikida, M. & Masumura, K.(1986).Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36, 396–398.[CrossRef] [Google Scholar]
  25. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2005).Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65397-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65397-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error