A novel methane-producing archaeon, strain NOBI-1 was isolated from an anaerobic, propionate-degradation enrichment culture, which was originally obtained from a mesophilic methanogenic sludge digesting municipal sewage sludge. Cells were non-motile, rod-shaped, 0.7–1.0 μm by 2.0 μm, and formed multicellular filaments longer than 8 μm. Growth was observed between 35 and 55 °C (optimum 50 °C) and pH 6.7 and 8.0 (optimum pH 7.0). The G+C content of the genomic DNA was 56.3 mol%. The strain utilized H and formate for growth and methane production. Based on comparative sequence analyses of the 16S rRNA gene and gene (encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane-production pathway), strain NOBI-1 was affiliated with the order , but it was significantly distant from any other known species within the order. The most closely related species based on 16S rRNA and gene sequence similarity were respectively ‘ Methanoregula boonei’ (93.7 % 16S rRNA gene sequence similarity) and (74.2 % deduced McrA amino acid sequence similarity to the type strain). These phenotypic and genetic properties justified the creation of a novel species of a new genus for the strain, for which we propose the name gen. nov., sp. nov. The type strain of is strain NOBI-1 (=DSM 16494 =JCM 12467 =NBRC 102358).


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Methane production from H by strain NOBI-1 . [PDF](169 KB)

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