A Gram-negative, aerobic, neutrophilic, moderately halophilic bacterial strain, HS225T, was isolated from seawater samples around the Zhoushan Archipelago, Zhejiang Province, China. The isolate grew optimally in media with 5.0 % NaCl, at pH 7.0–8.0 and at 25–30 °C. The predominant fatty acids were C16 : 0ω9c, C16 : 0, C12 : 0 3-OH and C18 : 1ω9c. The genomic DNA G+C content was 59.0 mol%. Based on 16S rRNA gene sequence analysis, the isolate was found to be affiliated to the genus Marinobacter. Strain HS225T exhibited closest phylogenetic affinity to Marinobacter koreensis DD-M3T (98.1 % 16S rRNA gene sequence similarity). DNA–DNA relatedness data and DNA G+C contents as well as physiological and biochemical test results allowed the genotypic and phenotypic differentiation of strain HS225T from closely related species. Therefore, it is proposed that strain HS225T represents a novel species of the genus Marinobacter, for which the name Marinobacter pelagius sp. nov. is proposed. The type strain is HS225T (=CGMCC 1.6775T =JCM 14804T)
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Supplementary Fig. S1. Scanning electron photomicrograph
of cells of strain HS225
Tgrown in HM broth. Bar, 2 µm.
Supplementary Figs S2 and S3. Phylogenetic trees based
on 16S rRNA gene sequences generated using the
maximum-parsimony method (Fig. S2) and the maximum-likelihood
method (Fig. S3).
[PDF](63 KB)
Supplementary Table S1. Fatty acid compositions of
strain HS225
Tand closely related Marinobacter type strains.
[PDF](17 KB)