A Gram-negative, aerobic, neutrophilic, moderately halophilic bacterial strain, HS225, was isolated from seawater samples around the Zhoushan Archipelago, Zhejiang Province, China. The isolate grew optimally in media with 5.0 % NaCl, at pH 7.0–8.0 and at 25–30 °C. The predominant fatty acids were C 9, C, C 3-OH and C 9. The genomic DNA G+C content was 59.0 mol%. Based on 16S rRNA gene sequence analysis, the isolate was found to be affiliated to the genus . Strain HS225 exhibited closest phylogenetic affinity to DD-M3 (98.1 % 16S rRNA gene sequence similarity). DNA–DNA relatedness data and DNA G+C contents as well as physiological and biochemical test results allowed the genotypic and phenotypic differentiation of strain HS225 from closely related species. Therefore, it is proposed that strain HS225 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HS225 (=CGMCC 1.6775 =JCM 14804)


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Scanning electron photomicrograph of cells of strain HS225 grown in HM broth. Bar, 2 µm.


Phylogenetic trees based on 16S rRNA gene sequences generated using the maximum-parsimony method (Fig. S2) and the maximum-likelihood method (Fig. S3). [PDF](63 KB)


Fatty acid compositions of strain HS225 and closely related Marinobacter type strains. [PDF](17 KB)


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