1887

Abstract

A Gram-negative, aerobic, neutrophilic, moderately halophilic bacterial strain, HS225, was isolated from seawater samples around the Zhoushan Archipelago, Zhejiang Province, China. The isolate grew optimally in media with 5.0 % NaCl, at pH 7.0–8.0 and at 25–30 °C. The predominant fatty acids were C 9, C, C 3-OH and C 9. The genomic DNA G+C content was 59.0 mol%. Based on 16S rRNA gene sequence analysis, the isolate was found to be affiliated to the genus . Strain HS225 exhibited closest phylogenetic affinity to DD-M3 (98.1 % 16S rRNA gene sequence similarity). DNA–DNA relatedness data and DNA G+C contents as well as physiological and biochemical test results allowed the genotypic and phenotypic differentiation of strain HS225 from closely related species. Therefore, it is proposed that strain HS225 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HS225 (=CGMCC 1.6775 =JCM 14804)

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2008-03-01
2019-12-09
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References

  1. Antunes, A., França, L., Rainey, F. A., Huber, R., Nobre, M. F., Edwards, K. J. & da Costa, M. S. ( 2007; ). Marinobacter salsuginis sp. nov., isolated from the brine–seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 57, 1035–1040.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. & Bertrand, J. C. ( 1992; ). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42, 568–576.[CrossRef]
    [Google Scholar]
  4. Gorshkova, N. M., Ivanova, E. P., Sergeev, A. F., Zhukova, N. V., Alexeeva, Y., Wright, J. P., Nicolau, D. V., Mikhailov, V. V. & Christen, R. ( 2003; ). Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53, 2073–2078.[CrossRef]
    [Google Scholar]
  5. Green, D. H., Bowman, J. P., Smith, E. A., Gutierrez, T. & Bolch, C. J. S. ( 2006; ). Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56, 523–527.[CrossRef]
    [Google Scholar]
  6. Gu, J., Cai, H., Yu, S.-L., Qu, R., Yin, B., Guo, Y.-F., Zhao, J.-Y. & Wu, X.-L. ( 2007; ). Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield. Int J Syst Evol Microbiol 57, 250–254.[CrossRef]
    [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  8. Huu, N. B., Denner, E. B. M., Ha, D. T. C., Wanner, G. & Stan-Lotter, H. ( 1999; ). Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49, 367–375.[CrossRef]
    [Google Scholar]
  9. Kim, B. Y., Weon, H. Y., Yoo, S. H., Kim, J. S., Kwon, S. W., Stackebrandt, E. & Go, S. J. ( 2006; ). Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56, 2653–2656.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  13. Liebgott, P. P., Casalot, L., Paillard, S., Lorquin, J. & Labat, M. ( 2006; ). Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 56, 2511–2516.[CrossRef]
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Márquez, M. C. & Ventosa, A. ( 2005; ). Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 and Marinobacter aquaeolei Nguyen et al. 1999 are heterotypic synonyms. Int J Syst Evol Microbiol 55, 1349–1351.[CrossRef]
    [Google Scholar]
  16. Martín, S., Márquez, M. C., Sánchez-Porro, C., Mellado, E., Arahal, D. R. & Ventosa, A. ( 2003; ). Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53, 1383–1387.[CrossRef]
    [Google Scholar]
  17. Mata, J. A., Martinez-Canovas, J., Quesada, E. & Bejar, V. ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  18. Romanenko, L. A., Schumann, P., Rohde, M., Zhukova, N. V., Mikhailov, V. V. & Stackebrandt, E. ( 2005; ). Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55, 143–148.[CrossRef]
    [Google Scholar]
  19. Shieh, W. Y., Jean, W. D., Lin, Y. T. & Tseng, M. ( 2003; ). Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49, 244–252.[CrossRef]
    [Google Scholar]
  20. Shivaji, S., Gupta, P., Chaturvedi, P., Suresh, K. & Delille, D. ( 2005; ). Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55, 1453–1456.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  23. Xu, X.-W., Wu, Y.-H., Wang, C.-S., Oren, A., Zhou, P. J. & Wu, M. ( 2007a; ). Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern of Zhe-Jiang, China. Int J Syst Evol Microbiol 57, 717–720.[CrossRef]
    [Google Scholar]
  24. Xu, X.-W., Wu, Y.-H., Zhou, Z., Wang, C.-S., Zhou, Y.-G., Zhang, H.-B., Wang, Y. & Wu, M. ( 2007b; ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57, 1619–1624.[CrossRef]
    [Google Scholar]
  25. Yoon, J. H., Shin, D. Y., Kim, I. G., Kang, K. H. & Park, Y. H. ( 2003; ). Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea. Int J Syst Evol Microbiol 53, 563–568.[CrossRef]
    [Google Scholar]
  26. Yoon, J. H., Yeo, S. H., Kim, I. G. & Oh, T. K. ( 2004; ). Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1799–1803.[CrossRef]
    [Google Scholar]
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Scanning electron photomicrograph of cells of strain HS225 grown in HM broth. Bar, 2 µm.

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Phylogenetic trees based on 16S rRNA gene sequences generated using the maximum-parsimony method (Fig. S2) and the maximum-likelihood method (Fig. S3). [PDF](63 KB)

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Fatty acid compositions of strain HS225 and closely related Marinobacter type strains. [PDF](17 KB)

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