1887

Abstract

A Gram-negative, psychrotolerant, chemoheterotrophic, aerobic, cream-coloured bacterium, designated IMCC3113, was isolated from coastal seawater from the Antarctic. On the basis of 16S rRNA gene sequence similarity analyses, the strain was most closely related to the type strains of (93.0 %) and (92.1 %) in the . Phylogenetic investigations using 16S rRNA gene sequences showed that this Antarctic marine isolate formed a robust monophyletic clade with the two species but constituted a distinct phyletic line in the clade. The DNA G+C content of strain IMCC3113 was 56.4 mol% and the major respiratory quinone was Q-9. Several phenotypic and physiological characteristics, including the temperature range and NaCl optimum for growth, several enzyme activities and the cellular fatty acid composition, served to differentiate the strain from the two species. Therefore strain IMCC3113 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC3113 (=KCCM 42675 =NBRC 102683).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65389-0
2008-02-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/353.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65389-0&mimeType=html&fmt=ahah

References

  1. Baik, K. S., Seong, C. N., Kim, E. M., Yi, H., Bae, K. S. & Chun, J. ( 2005; ). Hahella ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 55, 681–684.[CrossRef]
    [Google Scholar]
  2. Cho, J.-C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  3. Choo, Y.-J., Lee, K., Song, J. & Cho, J.-C. ( 2007; ). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. Int J Syst Evol Microbiol 57, 532–537.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Jeong, H., Yim, J. H., Lee, C., Choi, S. H., Park, Y. K., Yoon, S. H., Hur, C. G., Kang, H. Y., Kim, D. & other authors ( 2005; ). Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. Nucleic Acids Res 33, 7066–7073.[CrossRef]
    [Google Scholar]
  7. Jorgensen, J. H., Turnidge, J. D. & Washington, J. A. ( 1999; ). Antibacterial susceptibility tests: dilution and disk diffusion methods. In Manual of Clinical Microbiology, pp. 1526–1543. Edited by P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover & R. H. Yolken. Washington, DC: American Society for Microbiology.
  8. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Kim, D., Lee, J. S., Park, Y. K., Kim, J. F., Jeong, H., Oh, T. K., Kim, B. S. & Lee, C. H. ( 2007; ). Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396. J Appl Microbiol 102, 937–944.
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  11. Lee, H. K., Chun, J., Moon, E. Y., Ko, S. H., Lee, D. S., Lee, H. S. & Bae, K. S. ( 2001; ). Hahella chejuensis gen. nov., sp. nov., an extracellular-polysaccharide-producing marine bacterium. Int J Syst Evol Microbiol 51, 661–666.
    [Google Scholar]
  12. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology
  16. Swofford, D. ( 2002; ). paup*: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates.
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65389-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65389-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 353 - 356

Transmission electron micrographs of cells of strain IMCC3113 showing cell division and irregularly shaped rods and intracellular granules. [PDF](290 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error