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International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 5

sp. nov., a halophilic bacterium isolated from a saline lake on the Qinghai–Tibet Plateau, China Free

Abstract

A halophilic, Gram-negative bacterial strain, designated AJ261, which was isolated from a soil sample from a salt lake on the Qinghai–Tibet Plateau, was subjected to a polyphasic taxonomic study. The isolate grew optimally in the presence of 3–5 % NaCl and used various carbohydrates as sole carbon and energy sources. The genomic DNA G+C content was 63.0 mol%. The predominant fatty acids were C 7, C and C. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate had the highest sequence similarity with respect to type strains of (98.2 %), (98.1 %) and (97.2 %). The DNA–DNA relatedness of strain AJ261 with respect to NBRC 15536, CGMCC 1.2318 and DSM 5349 was 42, 25 and 26 %, respectively. Overall, the phenotypic, genotypic and phylogenetic results demonstrate that strain AJ261 represents a novel species within the genus , for which the name is proposed. The type strain is AJ261 (=CGMCC 1.6773 =JCM 14802).

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2008-05-01
2021-07-29
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References

  1. Arahal, D. R., Castillo, A. M., Ludwig, W., Schleifer, K. H. & Ventosa, A.(2002). Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst Appl Microbiol 25, 207–211.[CrossRef] [Google Scholar]
  2. Arahal, D. R., Vreeland, R. H., Litchfield, C. D., Mormile, M. R., Tindall, B. J., Oren, A., Bejar, V., Quesada, E. & Ventosa, A.(2007). Recommended minimal standards for describing new taxa of the family Halomonadaceae. Int J Syst Evol Microbiol 57, 2436–2446.[CrossRef] [Google Scholar]
  3. Ben Ali Gam, Z., Abdelkafi, S., Casalot, L., Tholozan, J. L., Oueslati, R. & Labat, M.(2007).Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend Dobson and Franzmann 1996 emend. Ntougias et al. 2007. Int J Syst Evol Microbiol 57, 2307–2313.[CrossRef] [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Dobson, S. J. & Franzmann, P. D.(1996). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef] [Google Scholar]
  6. Dobson, S. J., James, S. R., Franzmann, P. D. & McMeekin, T. A.(1990). Emended description of Halomonas halmophila (NCMB 1971T). Int J Syst Bacteriol 40, 462–463.[CrossRef] [Google Scholar]
  7. Euzéby, J. P.(1997). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  9. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  10. Franzmann, P. D., Wehmeyer, U. & Stackebrandt, E.(1989).Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11, 16–19. [Google Scholar]
  11. Garriga, M., Ehrmann, M. A., Arnau, J., Hugas, M. & Vogel, R. F.(1998).Carnimonas nigrificans gen. nov., sp. nov., a bacterial causative agent for black spot formation on cured meat products. Int J Syst Bacteriol 48, 677–686.[CrossRef] [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. Kuykendall, L. D., Roy, M. A., O’Neill, J. J. & Devine, T. E.(1988). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef] [Google Scholar]
  16. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  17. Martínez-Checa, F., Béjar, V., Martínez-Cánovas, M. J., Llamas, I. & Quesada, E.(2005).Halomonas almeriensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Cabo de Gata, Almería, south-east Spain. Int J Syst Evol Microbiol 55, 2007–2011.[CrossRef] [Google Scholar]
  18. Mata, J. A., Martinez-Canovas, J., Quesada, E. & Bejar, V.(2002). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef] [Google Scholar]
  19. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A.(1995). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef] [Google Scholar]
  20. Ntougias, S., Zervakis, G. I. & Fasseas, C.(2007).Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996. Int J Syst Evol Microbiol 57, 1975–1983.[CrossRef] [Google Scholar]
  21. Okamoto, T., Kuraishi, H., Nakamura, K., Ikenage, H., Kuraishi, H. & Yamasato, K.(1993).Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap. Arch Microbiol 160, 333–337. [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  24. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1982). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968. [Google Scholar]
  25. Ventosa, A., Gutiérrez, M. C., García, M. T. & Ruiz-Berraquero, F.(1989). Classification of “Chromobacterium marismortui” in a new genus, Chromohalobacter gen. nov., as Chromohalobacter marismortui comb. nov., nom. rev. Int J Syst Bacteriol 39, 382–386.[CrossRef] [Google Scholar]
  26. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E.(1980).Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef] [Google Scholar]
  27. Xu, X.-W., Ren, P.-G., Liu, S.-J., Wu, M. & Zhou, P.-J.(2005).Natrinema altunense sp. nov., an extremely halophilic archaeon isolated from a salt lake in Altun Mountain in Xinjiang, China. Int J Syst Evol Microbiol 55, 1311–1314.[CrossRef] [Google Scholar]
  28. Xu, X.-W., Wu, Y.-H., Zhou, Z., Wang, C.-S., Zhou, Y.-G., Zhang, H.-B., Wang, Y. & Wu, M.(2007).Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov., and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57, 1619–1624.[CrossRef] [Google Scholar]
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Halomonas caseinilytica sp. nov., a halophilic bacterium isolated from a saline lake on the Qinghai–Tibet Plateau, China
Int J Syst Evol Microbiol 58, 1259 (2008); https://doi.org/10.1099/ijs.0.65381-0
/content/journal/ijsem/10.1099/ijs.0.65381-0
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vol. , part 5, pp. 1259 - 1262

Fatty acid compositions (%) of strain AJ261 , NBRC 15536 and CGMCC 1.2318 .

Morphology of strain AJ261 as shown by Gram-stained cells grown in HM broth, transmission electron photomicrographs of cells grown on HM agar and scanning electron photomicrographs of cells grown in HM broth.

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