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Strain DCY16T, a Gram-positive, spore-forming, rod-shaped, motile bacterium, was isolated from soil and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY16T belonged to the genus Paenibacillus; highest sequence similarities were with Paenibacillus validus JCM 9077T (94.4 %), P. chinjuensis WN9T (94.4 %), P. naphthalenovorans DSM 14203T (94.2 %), P. ehimensis KCTC 3748T (92.8 %) and P. elgii KCTC 10016BPT (92.4 %). Chemotaxonomic data revealed that strain DCY16T possessed menaquinone MK-7 and the predominant fatty acids were C15 : 0 anteiso, C17 : 0 anteiso, C16 : 0 and C16 : 0 iso. The DNA G+C content of strain DCY16T was 50.9 mol%. Results of physiological and biochemical tests clearly demonstrated that strain DCY16T represents a distinct Paenibacillus species. Based on these data, DCY16T (=KCTC 13141T =JCM 14928T) should be classified as the type strain of a novel species, for which the name Paenibacillus ginsengihumi sp. nov. is proposed.
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 5, pp. 1164 - 1168
Supplementary Fig. S1. Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic relationships of strain DCY16 Tand type strains of all Paenibacillus species. [PDF](30 KB)