1887

Abstract

Biochemical and molecular genetic studies were performed on three isolates of an unknown Gram-positive, catalase-negative and rod-shaped organism isolated from the lungs and liver of two beaked whales. The organisms were tentatively identified as spp. based on cellular morphology and biochemical tests. 16S rRNA gene sequencing studies confirmed the provisional identification of the novel isolates as members of the genus , but the isolates did not correspond to any recognized species of this genus. The novel strains shared the same phenotypic characteristics and exhibited 100 % 16S rRNA gene sequence similarity. The nearest phylogenetic relatives of the novel isolates were DSM 16230 (94.2 % 16S rRNA gene sequence similarity), JCM 1047 (94.0 %), ATCC 700692 (94.0 %) and DSM 16049 (93.8 %). The novel isolates could be distinguished from these species and other related species of the genus by physiological and biochemical tests. On the basis of these phenotypic, physiological and phylogenetic findings, it is proposed that the new isolates from whales be classified as a novel species of the genus , sp. nov. The type strain is 142-2 (=CECT 7185=CCUG 53626).

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2008-04-01
2020-01-22
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References

  1. Aguirre, M. & Collins, M. D. ( 1993; ). Lactic acid bacteria and human clinical infection. J Appl Bacteriol 75, 95–107.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. DSMZ ( 20001; ). Catalogue of Strains, 7th edn, p. 617. Braunschweig: DSMZ. http://www.dsmz.de/species/murein.htm
  4. Edwards, C. G., Collins, M. D., Lawson, P. A. & Rodriguez, A. V. ( 2000; ). Lactobacillus nagelii sp. nov., an organism isolated from a partially fermented wine. Int J Syst Evol Microbiol 50, 699–702.[CrossRef]
    [Google Scholar]
  5. Endo, A. & Okada, S. ( 2005; ). Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55, 83–85.[CrossRef]
    [Google Scholar]
  6. Euzéby, J. P. ( 1997; ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592 (Last full update May 03, 2007).[CrossRef]
    [Google Scholar]
  7. Felis, G. E. & Dellaglio, F. ( 2007; ). Taxonomy of Lactobacilli and Bifidobacteria. Curr Issues Intest Microbiol 8, 44–61.
    [Google Scholar]
  8. Garrity, G. M., Bell, J. A. & Lilburn, T. G. ( 2004; ). Taxonomic outline of the prokaryotes. In Bergey's Manual of Systematic Bacteriology, 2nd edn, release 5.0. New York: Springer. DOI:10.1007/bergeysoutline200405
  9. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  10. Hammes, W. P., Weiss, N. & Holzapfel, W. ( 1992; ). The genera Lactobacillus and Carnobacterium. In The Prokaryotes, 2nd edn, pp. 1535–1594. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  11. Kandler, O. & Weiss, N. ( 1986; ). Genus Lactobacillus Beijerinck 1901, 212AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1209–1234. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  12. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  14. Morotomi, M., Yuki, N., Kado, Y., Kushiro, A., Shimazaki, T., Watanabe, K. & Yuyama, T. ( 2002; ). Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses. Int J Syst Evol Microbiol 52, 211–214.
    [Google Scholar]
  15. Osawa, R., Fujisawa, T. & Pukall, R. ( 2006; ). Lactobacillus apodemi sp. nov., a tannase-producing species isolated from wild mouse faeces. Int J Syst Evol Microbiol 56, 1693–1696.[CrossRef]
    [Google Scholar]
  16. Page, R. D. M. ( 1996; ). treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  17. Pearson, W. R. ( 1994; ). Using the fasta program to search protein and DNA sequence databases. Methods Mol Biol 24, 307–331.
    [Google Scholar]
  18. Pedersen, C. & Roos, S. ( 2004; ). Lactobacillus saerimneri sp. nov., isolated from pig faeces. Int J Syst Evol Microbiol 54, 1365–1368.[CrossRef]
    [Google Scholar]
  19. Rasmussen, S. W. ( 1995; ). DNA Tools, a Software Package for DNA Sequence Analysis. Copenhagen: Carlsberg Laboratory.
  20. Rodas, A. M., Chenoll, E., Macián, M. C., Ferrer, S., Pardo, I. & Aznar, R. ( 2006; ). Lactobacillus vini sp. nov., a wine lactic acid bacterium homofermentative for pentoses. Int J Syst Evol Microbiol 56, 513–517.[CrossRef]
    [Google Scholar]
  21. Roos, S., Karner, F., Axelsson, L. & Jonsson, H. ( 2000; ). Lactobacillus muscosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. Int J Syst Evol Microbiol 50, 251–258.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  24. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Tanasupawat, S., Shida, O., Okada, S. & Komagata, K. ( 2000; ). Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485.[CrossRef]
    [Google Scholar]
  27. Vela, A. I., Goyache, J., Tarradas, C., Luque, I., Mateos, A., Moreno, M. A., Borge, C., Perea, J. A., Dominguez, L. & other authors ( 2003; ). Analysis of genetic diversity of Streptococcus suis clinical isolates from pigs in Spain by pulsed-field gel electrophoresis. J Clin Microbiol 41, 2498–2502.[CrossRef]
    [Google Scholar]
  28. Vela, A. I., Collins, M. D., Lawson, P. A., García, N., Domínguez, L. & Fernández-Garayzábal, J. F. ( 2005; ). Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. Int J Syst Evol Microbiol 55, 643–647.[CrossRef]
    [Google Scholar]
  29. Wibowo, D., Eschenbruch, R., Davis, C. R., Fleet, G. H. & Lee, T. H. ( 1985; ). Occurrence and growth of lactic acid bacteria in wine: a review. Am J Enol Vitic 36, 302–313.
    [Google Scholar]
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