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Abstract

A Gram-negative, heterotrophic, agarolytic, marine bacterium, designated strain SA1, was isolated from a seawater sample collected in the shallow coastal region of Keelung, Taiwan. Cells were straight to slightly curved rods. Nearly all of the cells were non-motile and non-flagellated during the exponential phase of growth in broth cultures; a few cells (<1 %) were motile and were considered to have monotrichous flagella. The isolate required NaCl for growth and grew optimally at 30–35 °C and 2–3 % (w/v) NaCl. It grew aerobically and was incapable of anaerobic growth by fermentation of glucose or other carbohydrates. However, anaerobic growth could be achieved by reduction of nitrate to nitrite. Polar lipids comprised phosphatidylethanolamine (71.8 %), diphosphatidylglycerol (12.7 %), phosphatidylglycerol (12.2 %) and phosphatidylserine (3.3 %). Isoprenoid quinones consisted of Q-10 (87.5 %), MK-9 (6.6 %) and MK-7 (5.9 %). Major cellular fatty acids were C 7 and/or iso-C 2-OH (28.6 %), C 8 (22.8 %), C (14.5 %), C 7 (11.0 %) and C (6.4 %). The DNA G+C content was 55.6 mol%. Phylogeny based on 16S rRNA gene sequence analysis showed that strain SA1 formed a distinct lineage within the class . Strain SA1 was related most closely to , spp., , spp. and spp., strains of these species sharing <93 % 16S rRNA gene sequence similarity with strain SA1. The phylogenetic data and those from physiological, morphological and chemotaxonomic characterizations indicated that strain SA1 represents a novel species and genus, for which the name gen. nov., sp. nov. is proposed. The type strain is SA1 (=BCRC 17597=JCM 13881).

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2008-04-01
2024-12-03
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References

  1. Chiu, H.-H., Shieh, W. Y., Lin, S. Y., Tseng, C.-M., Chiang, P.-W. & Wagner-Döbler, I.(2007).Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary. Int J Syst Evol Microbiol 57, 1209–1216.[CrossRef] [Google Scholar]
  2. Distel, D. L., Morrill, W., Maclaren-Toussaint, N., Franks, D. & Waterbury, J.(2002).Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int J Syst Evol Microbiol 52, 2261–2269.[CrossRef] [Google Scholar]
  3. Ekborg, N. A., Gonzalez, J. M., Howard, M. B., Taylor, L. E., Hutcheson, S. W. & Weiner, R. M.(2005).Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol 55, 1545–1549.[CrossRef] [Google Scholar]
  4. Gonzàlez, J. M. & Weiner, R. M.(2000). Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides. Int J Syst Evol Microbiol 50, 831–834.[CrossRef] [Google Scholar]
  5. Hendricks, C. W., Doyle, J. D. & Hugley, B.(1995). A new solid medium for enumerating cellulose-utilizing bacteria in soil. Appl Environ Microbiol 61, 2016–2019. [Google Scholar]
  6. Hobel, C. F. V., Marteinsson, V. T., Hreggvidsson, G. O. & Kristjansson, J. K.(2005). Investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16S rRNA and chitinase genes. Appl Environ Microbiol 71, 2771–2776.[CrossRef] [Google Scholar]
  7. Jean, W. D., Shieh, W. Y. & Chiu, H.-H.(2006a).Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae. Int J Syst Evol Microbiol 56, 899–905.[CrossRef] [Google Scholar]
  8. Jean, W. D., Shieh, W. Y. & Liu, T. Y.(2006b).Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 56, 1245–1250.[CrossRef] [Google Scholar]
  9. Lin, Y.-T. & Shieh, W. Y.(2006).Zobellella denitrificans gen. nov., sp. nov. and Zobellella taiwanensis sp. nov., two denitrifying bacteria capable of fermentative metabolism. Int J Syst Evol Microbiol 56, 1209–1215.[CrossRef] [Google Scholar]
  10. Nishijima, M., Sakai, M. & Sano, H.(1997). Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28, 113–122.[CrossRef] [Google Scholar]
  11. Sasser, M.(1997).Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  12. Scheuermayer, M., Gulder, T. A., Bringmann, G. & Hentschel, U.(2006).Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum ‘Verrucomicrobia’, isolated from a sponge (Porifera). Int J Syst Evol Microbiol 56, 2119–2124.[CrossRef] [Google Scholar]
  13. Shieh, W. Y. & Jean, W. D.(1998).Alterococcus agarolyticus gen. nov., sp. nov., a halophilic thermophilic bacterium capable of agar degradation. Can J Microbiol 44, 637–645.[CrossRef] [Google Scholar]
  14. Shieh, W. Y. & Liu, C. M.(1996). Denitrification by a novel halophilic fermentative bacterium. Can J Microbiol 42, 507–514.[CrossRef] [Google Scholar]
  15. Shieh, W. Y., Chen, A.-L. & Chiu, H.-H.(2000).Vibrio aerogenes sp. nov., a facultatively anaerobic, marine bacterium that ferments glucose with gas production. Int J Syst Evol Microbiol 50, 321–329.[CrossRef] [Google Scholar]
  16. Shieh, W. Y., Lin, Y.-T. & Jean, W. D.(2004).Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54, 2307–2312.[CrossRef] [Google Scholar]
  17. Yoon, J., Ishikawa, S., Kasai, H. & Yokota, A.(2007).Persicitalea jodogahamensis gen. nov., sp. nov., a marine bacterium of the family ‘Flexibacteraceae’, isolated from seawater in Japan. Int J Syst Evol Microbiol 57, 1014–1017.[CrossRef] [Google Scholar]
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vol. , part 4, pp. 895 – 900

Maximum-likelihood tree showing the phylogenetic relationship between strain SA1 and related bacteria in the class [ PDF] (53 KB)



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