RT Journal Article SR Electronic(1) A1 Jeon, Che Ok A1 Lim, Jee-Min A1 Jang, Ho Hee A1 Park, Dong-Jin A1 Xu, Li-Hua A1 Jiang, Cheng-Lin A1 Kim, Chang-JinYR 2008 T1 Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 10 SP 2282 OP 2286 DO https://doi.org/10.1099/ijs.0.65369-0 PB Microbiology Society, SN 1466-5034, AB Two Gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5–7 % (w/v) NaCl were isolated from a salt lake, Xin-Jiang Province, China. The strains, designated BH312T and BH314, grew in the presence of 1–18 % (w/v) NaCl and at temperatures of 15–50 °C (optimum: 40 °C) and pH values of 5.5–10.0 (optimum: pH 7.5–8.0). The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 38.8–39.0 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates formed a cluster with Gracilibacillus orientalis XH-63T within the genus Gracilibacillus. The levels of 16S rRNA gene sequence similarity with closely related type strains were lower than 96.2 %. On the basis of phylogenetic, phenotypic and chemotaxonomic properties, the isolates represent a novel species of the genus Gracilibacillus for which the name Gracilibacillus lacisalsi sp. nov. is proposed. The type strain is BH312T (=KCTC 13129T=DSM 19029T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65369-0