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Abstract

An orange-coloured bacterial strain, designated 5715S-11, was isolated from an air sample in Suwon, Republic of Korea. The strain was strictly aerobic, Gram-negative, non-spore-forming, non-flagellated and rod-shaped, frequently forming filaments. Growth of the strain was observed at 4–40 °C (optimum, 30 °C), pH 6.0–8.0 (optimum, pH 7.0) and 0–2 % (w/v) NaCl. Phylogenetically, strain 5715S-11 was shown to be a member of the family within the phylum . Its closest relatives were LMG 10896 (87.9 % 16S rRNA gene sequence similarity) and LMG 22510 (86.2 % 16S rRNA gene sequence similarity). Predominant cellular fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH), C 5 and iso-C. Major polar lipids were phosphatidylethanolamine and unknown aminolipids and polar lipids. On the basis of the evidence from this polyphasic study, strain 5715S-11 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 5715S-11 (=KACC 12603 =DSM 19387).

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2008-02-01
2024-12-05
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K.(1987).Current Protocols in Molecular Biology. New York: Wiley.
  2. Breznak, J. A. & Costilow, R. N.(1994). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  3. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  4. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  5. Kwon, S.-W., Kim, J.-S., Park, I.-C., Yoon, S.-H., Park, D.-H., Lim, C.-K. & Go, S.-J.(2003).Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef] [Google Scholar]
  6. Larkin, J. M. & Borrall, R.(1978).Spirosomaceae, a new family to contain the genera Spirosoma Migula 1894, Flectobacillus Larkin et al. 1977, and Runella Larkin and Williams 1978. Int J Syst Bacteriol 28, 595–596.[CrossRef] [Google Scholar]
  7. Larkin, J. M. & Borrall, R.(1984). Family I. Spirosomaceae Larkin and Borrall 1978, 595AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 125–126. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  8. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K. H.(1998). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef] [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  10. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  11. Reichenbach, H.(1992). The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  12. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  13. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  16. Vancanneyt, M., Nedashkovskaya, O. I., Snauwaert, C., Mortier, S., Vandemeulebroecke, K., Hoste, B., Dawyndt, P., Frolova, G. M., Janssens, D. & Swings, J.(2006).Larkinella insperata gen. nov., sp. nov., a bacterium of the phylum ‘Bacteroidetes’ isolated from water of a steam generator. Int J Syst Evol Microbiol 56, 237–241.[CrossRef] [Google Scholar]
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vol. , part 2, pp. 474 - 478

Cellular fatty acid contents of strain 5715S-11 and the type strains of and . [PDF](38 KB)



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