1887

Abstract

A yellow-pigmented, Gram-negative, aerobic bacterial strain comprising rod-shaped cells devoid of flagellar and gliding motility, designated strain JC2129, was isolated from tidal flat sediment of Dongmak on Ganghwa Island, South Korea. Results from a 16S rRNA gene sequence analysis indicated that the isolate belonged to the family ; the highest level of nucleotide sequence similarity (91.9 %) occurred with DSW-5. The predominant cellular fatty acids were iso-C (19.8 %), iso-C G (14.0 %), iso-C 3-OH (13.7 %) and iso-C (6.4 %). Flexirubin-type pigments were absent. The major isoprenoid quinone was MK-6. The DNA G+C content was 43–45 mol%. Data from a polyphasic taxonomy study suggested that the isolate represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is JC2129 (=KCTC 12704 =JCM 14002).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65346-0
2008-01-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/139.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65346-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  2. Choi, D. H. & Cho, B. C.(2006).Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 771–776.[CrossRef] [Google Scholar]
  3. Choi, D. H., Kim, Y. G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C.(2006).Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef] [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  5. Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366. [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(2005).phylip (phylogeny inference package), version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N.(2004).Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef] [Google Scholar]
  9. Gosink, J. J., Woese, C. R. & Staley, J. T.(1998).Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef] [Google Scholar]
  10. Hsu, S. C. & Lockwood, J. L.(1975). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29, 422–426. [Google Scholar]
  11. Jeon, Y.-S., Chung, H., Park, S., Hur, I., Lee, J.-H. & Chun, J.(2005). jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef] [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Kim, B. S., Oh, H. M., Kang, H., Park, S. S. & Chun, J.(2004). Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol Biotechnol 14, 205–211. [Google Scholar]
  14. Kim, B. S., Oh, H. M., Kang, H. & Chun, J.(2005). Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol 43, 144–151. [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  16. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  17. Lyman, J. & Fleming, R. H.(1940). Composition of sea water. J Mar Res 3, 134–146. [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Washington, DC: American Society for Microbiology.
  22. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S.(2001). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef] [Google Scholar]
  23. Weeks, O. B.(1981). Preliminary studies of the pigments of Flavobacteriumbreve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium–Cytophaga Group, pp. 108–114. Edited by O. B. Weeks. Weinheim: Gesellschaft für Biotechnologische Forschung.
  24. Yi, H. & Chun, J.(2006).Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44, 171–176. [Google Scholar]
  25. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2005).Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65346-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65346-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 139 - 143

Transmission electron micrograph of a negatively stained cell of strain JC2129 grown on MA at 37 °C for 3 days. Bar, 0.5 µm.



IMAGE
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error