RT Journal Article SR Electronic(1) A1 Wagner, Isaac D. A1 Zhao, Weidong A1 Zhang, Chuanlun L. A1 Romanek, Christopher S. A1 Rohde, Manfred A1 Wiegel, JuergenYR 2008 T1 Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 11 SP 2565 OP 2573 DO https://doi.org/10.1099/ijs.0.65343-0 PB Microbiology Society, SN 1466-5034, AB Several strains of heterotrophic, anaerobic thermophilic bacteria were isolated from hot springs of the Uzon Caldera, Kamchatka, Far East Russia. Strain JW/IW010T was isolated from a hot spring within the West sector of the Eastern Thermal field, near Pulsating Spring in the Winding Creek area. Cells of strain JW/IW010T were straight to slightly curved rods, 0.5 μm in width and variable in length from 2 to 5 μm and occasionally up to 15 μm, and formed oval subterminal spores. Cells stained Gram-negative, but were Gram-type positive. Growth was observed between 32.5 and 69 °C with an optimum around 61 °C (no growth occurred at or below 30 °C, or at or above 72 °C). The pH60 °C range for growth was 4.2–8.9 with an optimum at 7.1 (no growth occurred at or below pH60 °C 3.9, or at 9.2 or above). The shortest observed doubling-time at pH60 °C 6.9 and 61 °C was 30 min. Strain JW/IW010T was chemo-organotrophic; yeast extract, peptone, Casamino acids and tryptone supported growth. Yeast extract was necessary for the utilization of non-proteinaceous substrates, and growth was observed with inulin, cellobiose, maltose, sucrose, glucose, fructose, galactose, mannose, xylose, trehalose, mannitol, pyruvate and crotonate. The G+C content of the genomic DNA of strain JW/IW010T was 33.6 mol% (HPLC method). The major phospholipid fatty acids were iso-15 : 0 (53.5 %), 15 : 0 (11.8 %), 16 : 0 (7.3 %), 10-methyl 16 : 0 (7.3 %) and anteiso-15 : 0 (5.3 %). 16S rRNA gene sequence analysis placed strain JW/IW010T in the genus Thermoanaerobacter of the family ‘Thermoanaerobacteriaceae’ (Firmicutes), with Thermoanaerobacter sulfurigignens JW/SL-NZ826T (97 % 16S rRNA gene sequence similarity) and Thermoanaerobacter kivui DSM 2030T (94.5 %) as the closest phylogenetic relatives with validly published names. The level of DNA–DNA relatedness between strain JW/IW010T and Thermoanaerobacter sulfurigignens JW/SL-NZ826T was 64 %. Based on the physiological, phylogenetic and genotypic data, strain JW/IW010T represents a novel taxon, for which the name Thermoanaerobacter uzonensis sp. nov. is proposed. The type strain is JW/IW010T (=ATCC BAA-1464T=DSM 18761T). The effectively published strain, 1501/60, of ‘Clostridium uzonii’ [ Krivenko, V. V., Vadachloriya, R. M., Chermykh, N. A., Mityushina, L. L. & Krasilnikova, E. N. (1990). Microbiology (English translation of Mikrobiologiia) 59, 741–748 ] had approximately 88.0 % DNA–DNA relatedness with strain JW/IW010T and was included in the novel taxon., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65343-0