1887

Abstract

A Gram-positive or Gram-variable, motile, moderately halophilic, rod-shaped bacterial strain, ISL-50, was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic study. Strain ISL-50 grew optimally at pH 7.0–8.0 and 37 °C in the presence of 8 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-50 belonged to the genus . Strain ISL-50 exhibited 16S rRNA gene sequence similarity values of 95.3–98.2 % with respect to the type strains of recognized species. The cell-wall peptidoglycan was based on -diaminopimelic acid, the predominant menaquinone was MK-7 and the major fatty acids were anteiso-C and anteiso-C. The DNA G+C content was 39.3 mol%. DNA–DNA relatedness data and differential phenotypic properties revealed that strain ISL-50 was distinguishable from the recognized species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ISL-50 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ISL-50 (=KCTC 13145=CCUG 54358).

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2008-03-01
2021-12-06
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References

  1. Abd El-Rahman, H. A., Fritze, D., Sproer, C. & Claus, D.(2002). Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. Int J Syst Evol Microbiol 52, 2127–2133.[CrossRef] [Google Scholar]
  2. Amoozegar, M. A., Malekzadeh, F., Malik, K. A., Schumann, P. & Spröer, C.(2003).Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53, 1059–1063.[CrossRef] [Google Scholar]
  3. An, S.-Y., Asahara, M., Goto, K., Kasai, H. & Yokota, A.(2007a).Terribacillus saccharophilus gen. nov., sp. nov. and Terribacillus halophilus sp. nov., spore-forming bacteria isolated from field soil in Japan. Int J Syst Evol Microbiol 57, 51–55.[CrossRef] [Google Scholar]
  4. An, S.-Y., Kanoh, K., Kasai, H., Goto, K. & Yokota, Y.(2007b).Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57, 2476–2479.[CrossRef] [Google Scholar]
  5. Baumann, P. & Baumann, L.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  6. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  7. Claus, D., Fahmy, F., Rolf, H. J. & Tosunoglu, N.(1983).Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4, 496–506.[CrossRef] [Google Scholar]
  8. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y.(1957). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49, 25–68.[CrossRef] [Google Scholar]
  9. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  11. Hua, N.-P., Kanekiyo, A., Fujikura, K., Yasuda, H. & Naganuma, T.(2007).Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57, 1243–1249.[CrossRef] [Google Scholar]
  12. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  13. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  14. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  15. Liu, W. Y., Zeng, J., Wang, L., Dou, Y. T. & Yang, S. S.(2005).Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55, 1991–1996.[CrossRef] [Google Scholar]
  16. Nunes, I., Tiago, I., Pires, A. L., da Costa, M. S. & Veríssimo, A.(2006).Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil. Int J Syst Evol Microbiol 56, 1841–1845.[CrossRef] [Google Scholar]
  17. Rheims, H., Fruhling, A., Schumann, P., Rohde, M. & Stackebrandt, E.(1999).Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49, 795–802.[CrossRef] [Google Scholar]
  18. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behaviour of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef] [Google Scholar]
  19. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI.
  20. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  21. Soto-Ramírez, N., Sánchez-Porro, C., Rosas-Padilla, S., Almodóvar, K., Jiménez, G., Machado-Rodríguez, M., Zapata, M., Ventosa, A. & Montalvo-Rodríguez, R.(2008).Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans. Int J Syst Evol Microbiol 58, 125–130.[CrossRef] [Google Scholar]
  22. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H.(1996).Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef] [Google Scholar]
  23. Staley, J. T.(1968).Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942. [Google Scholar]
  24. Takeuchi, M. & Hatano, K.(1998). Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. Int J Syst Bacteriol 48, 973–982.[CrossRef] [Google Scholar]
  25. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  27. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  28. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  29. Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef] [Google Scholar]
  30. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2004).Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8, 23–28.[CrossRef] [Google Scholar]
  31. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2005).Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55, 2413–2417.[CrossRef] [Google Scholar]
  32. Yoon, J.-H., Kang, S.-J., Jung, Y.-T. & Oh, T.-K.(2007).Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus. Int J Syst Evol Microbiol 57, 2021–2025.[CrossRef] [Google Scholar]
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vol. , part 3, pp. 622 – 627

Transmission electron micrograph of sp. nov. ISL-50 from exponentially growing cultures. Bar, 1 µm.



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