Four bacterial strains, designated K9, K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a medium containing 12–120 mM n-butanol. The strains were strictly aerobic, Gram-positive endospore-formers. The best growth was achieved at 25 °C and pH 7.0 in medium containing 1 % (w/v) NaCl. The strains showed identical profiles of 16S–23S rRNA internal transcribed spacer PCR and nearly identical 16S rRNA gene PCR-RFLP electrophoretic patterns and physiological characteristics, demonstrating their relationship at the species level. The cellular fatty acid profile of K9 consisted of significant amounts of the C branched-chain fatty acids iso-C (16.78 %) and anteiso-C (45.80 %). The diagnostic cell-wall diamino acid was -diaminopimelic acid. The 16S rRNA gene sequence of K9 showed the highest similarity to the sequences of DSM 1321 and LMG 20238 (98.3 and 97.7 %, respectively). The DNA G+C content was 37.4 mol%. Studies of DNA–DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strains K9, K105, K1012A and K101 to be described as members of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K9 (=DSM 18926 =LMG 23974).


Article metrics loading...

Loading full text...

Full text loading...



  1. Arp, D. J.(1999). Butane metabolism by butane-grown ‘Pseudomonas butanovora’. Microbiology 145, 1173–1180.[CrossRef] [Google Scholar]
  2. Birnboim, H. C. & Doly, J.(1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513–1523.[CrossRef] [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  4. Claus, D. & Berkeley, R. C. W.(1986). Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  5. Daffonchio, D., Borin, S., Frova, G., Manachini, P. L. & Sorlini, C.(1998). PCR fingerprinting of whole genomes: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis. Int J Syst Bacteriol 48, 107–116.[CrossRef] [Google Scholar]
  6. Heyrman, J., Logan, N. A., Rodríguez-Díaz, M., Scheldeman, P., Lebbe, L., Swings, J., Heyndrickx, M. & De Vos, P.(2005). Study of mural painting isolates leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int J Syst Evol Microbiol 55, 119–131.[CrossRef] [Google Scholar]
  7. Kuisiene, N., Jomantiene, R., Valiunas, D. & Chitavichius, D.(2002). Characterization of thermophilic proteolytic spore-forming bacteria from a geothermal site in Lithuania based on 16S rDNA RFLP and ITS-PCR analyses. Microbiology English translation of Mikrobiologiia 71, 712–716.[CrossRef] [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  9. Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Eggink, G. & Witholt, B.(1988). Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl Environ Microbiol 54, 2924–2932. [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  11. MIDI(1999).Sherlock Microbial Identification System, Operating Manual, version 3.0. Newark, DE: MIDI Inc.
  12. Mignot, T., Denis, B., Couture-Tosi, E., Kolstø, A.-B., Mock, M. & Fouet, A.(2001). Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure. Environ Microbiol 3, 493–501.[CrossRef] [Google Scholar]
  13. Priest, F. G., Goodfellow, M. & Todd, C.(1988). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882. [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Schleifer, K.-H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  17. Veeranagouda, Y., Vijaykumar, M. H., Patil, N. K., Nayak, A. S. & Karegoudar, T. B.(2006). Degradation of 1-butanol by solvent-tolerant Enterobacter sp. VKGH12. Int Biodeterior Biodegrad 57, 186–189.[CrossRef] [Google Scholar]
  18. Yumoto, I., Hirota, K., Yamaga, S., Nodasaka, Y., Kawasaki, T., Matsuyama, H. & Nakajima, K.(2004).Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54, 1997–2001.[CrossRef] [Google Scholar]

Data & Media loading...


vol. , part 2, pp. 505 - 509

ARDRA ( III) and RISA gel-electrophoretic profiles of strains K9 , K105, K1012A and K101.

ARDRA ( I, II, I) gel-electrophoretic profiles of strains K9 , K105, K1012A and K101.

[PDF file of Supplementary Figs S1 and S2](163 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error