An extremely halophilic archaeon was isolated from a sample of the brine–sediment interface of the Shaban Deep in the northern Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to (99.3 %), the sole species of the genus . Strain SARL4B formed non-pigmented colonies and showed optimum growth at 45 °C, in 27 % (w/v) NaCl and at pH 6.5–7.0. This organism utilized a few complex substrates, such as yeast extract and starch, for growth. Strain SARL4B grew under anaerobic and microaerophilic conditions but grew extremely poorly under aerobic conditions. The ether lipids were diphytanyl derivatives. The DNA G+C content of the type strain was 61.7 mol%. On the basis of the phylogenetic data and physiological and biochemical characteristics, strain SARL4B represents a novel species of the genus , for which the name is proposed. The type strain is SARL4B (=DSM 18392=JCM 14471). An emended description of the genus is also proposed.


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vol. , part 1, pp. 215 - 220

Polar lipid profiles of and strain SARL4B separated by mono-dimensional TLC. [ PDF] (106 KB)


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