%0 Journal Article %A Kharroub, K. %A Jiménez-Pranteda, M. L. %A Aguilera, M. %A Boulahrouf, A. %A Ramos-Cormenzana, A. %A Monteoliva-Sánchez, M. %T Halomonas sabkhae sp. nov., a moderately halophilic bacterium isolated from an Algerian sabkha %D 2008 %J International Journal of Systematic and Evolutionary Microbiology, %V 58 %N 1 %P 40-44 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65307-0 %I Microbiology Society, %X A novel moderately halophilic bacterium belonging to the genus Halomonas was isolated from brine samples collected from Ezzemoul sabkha in north-eastern Algeria. The cells of strain 5-3T were Gram-negative, rod-shaped and non-motile. The strain was catalase- and oxidase-positive and produced an exopolysaccharide. Growth occurred at NaCl concentrations of 5–25 % (optimum at 7.5 %), at 30–50 °C (optimum at 37–40 °C) and at pH 6.0–9.0 (optimum at pH 7.5). The major fatty acids were C12 : 0 3-OH, C16 : 1 ω7c/iso-C15 : 0 2-OH, C16 : 0, C18 : 1 ω7c and C19 : 0 ω8c cyclo. The G+C content of the genomic DNA was 57.0 mol% (T m). The affiliation of strain 5-3T with the genus Halomonas was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was Halomonas halmophila, which showed a 16S rRNA gene sequence similarity of 99.7 %. However, the level of DNA–DNA relatedness between the novel isolate and the related Halomonas species was less than 31.4 %. On the basis of the data from this polyphasic study, strain 5-3T represents a novel species of the genus Halomonas, for which the name Halomonas sabkhae sp. nov. is proposed. The type strain is 5-3T (=CECT 7246T=DSM 19122T=LMG 24084T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65307-0