A novel moderately halophilic bacterium belonging to the genus was isolated from brine samples collected from Ezzemoul sabkha in north-eastern Algeria. The cells of strain 5-3 were Gram-negative, rod-shaped and non-motile. The strain was catalase- and oxidase-positive and produced an exopolysaccharide. Growth occurred at NaCl concentrations of 5–25 % (optimum at 7.5 %), at 30–50 °C (optimum at 37–40 °C) and at pH 6.0–9.0 (optimum at pH 7.5). The major fatty acids were C 3-OH, C 7/iso-C 2-OH, C, C 7 and C 8 cyclo. The G+C content of the genomic DNA was 57.0 mol% ( ). The affiliation of strain 5-3 with the genus was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was , which showed a 16S rRNA gene sequence similarity of 99.7 %. However, the level of DNA–DNA relatedness between the novel isolate and the related species was less than 31.4 %. On the basis of the data from this polyphasic study, strain 5-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5-3 (=CECT 7246=DSM 19122=LMG 24084).


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vol. , part 1, pp. 40 - 44

Maximum-parsimony phylogenetic tree, based on 16S rRNA gene sequences, showing relationships between strain 5-3 and species of the genus plus other taxa comprising Gram-negative, halophilic bacteria. [ PDF] (31 KB)


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