1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 4

gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan Free

Abstract

A strictly anaerobic, mesophilic, sulfate-reducing bacterial strain, designated MSL71, was isolated from an estuarine sediment from the Sea of Japan bordering the Japanese islands and was characterized phenotypically and phylogenetically. The cells were found to be Gram-negative, motile, non-spore-forming, slightly curved rods. Catalase and oxidase activities were not detected. The optimum NaCl concentration for growth was 2.0 % (w/v), the optimum temperature was 30 °C and the optimum pH was 6.3. Strain MSL71 utilized formate, butyrate, pyruvate, lactate, malate, ethanol, propanol, butanol, glycerol and H as electron donors for sulfate reduction. The organic electron donors used were incompletely oxidized, mainly to acetate. The strain did not use acetate, propionate, fumarate, succinate, methanol, glycine, alanine, serine, aspartate or glutamate. Sulfite and thiosulfate were used as electron acceptors with lactate as an electron donor, but fumarate was not utilized. Without electron acceptors, pyruvate and malate, but not lactate or fumarate, were fermented. The genomic DNA G+C content was 62.0 mol%. Menaquinone MK-8(H) was the major respiratory quinone. The major cellular fatty acids were C, C, C 7, C 9, C 7 and C 3-OH. A phylogenetic analysis based on the 16S rRNA gene sequence placed the strain in the class . The closest recognized relative of strain MSL71 was (93.9 % sequence similarity) and the next closest recognized species was (93.5 %). On the basis of the significant differences in the 16S rRNA gene sequence and phenotypic characteristics between strain MSL71 and each of the related species, a novel genus and species, gen. nov., sp. nov., are proposed to accommodate strain MSL71. The type strain is MSL71 (=JCM 14721=DSM 19427).

  • Published Online:
Keyword(s): CFA, whole-cell fatty acid and SRB, sulfate-reducing bacteria
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65306-0
2008-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/4/826.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65306-0&mimeType=html&fmt=ahah

References

  1. Akasaka, H., Izawa, T., Ueki, K. & Ueki, A.(2003a). Phylogeny of numerically abundant culturable anaerobic bacteria associated with degradation of rice plant residue in Japanese paddy field soil. FEMS Microbiol Ecol 43, 149–161.[CrossRef] [Google Scholar]
  2. Akasaka, H., Ueki, A., Hanada, S., Kamagata, Y. & Ueki, K.(2003b).Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int J Syst Evol Microbiol 53, 1991–1998.[CrossRef] [Google Scholar]
  3. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  4. Blenden, D. C. & Goldberg, H. S.(1965). Silver impregnation stain for Leptospira and flagella. J Bacteriol 89, 899–900. [Google Scholar]
  5. Castro, H. F., Williams, N. H. & Ogram, A.(2000). Phylogeny of sulfate-reducing bacteria. FEMS Microbiol Ecol 31, 1–9. [Google Scholar]
  6. Felsenstein, J.(2006).phylip (phylogeny inference package), version 3.66. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Hansen, T. A.(1993). Carbon metabolism in sulfate-reducing bacteria. In The Sulfate-Reducing Bacteria, pp. 21−40. Edited by J. M. Odom & R. Singleton. New York: Springer.
  8. Hungate, R. E.(1966).The Rumen and its Microbes. New York: Academic Press.
  9. Jørgensen, B. B.(1982). Mineralization of organic matter in the sea bed – the role of sulphate reduction. Nature 296, 643–645.[CrossRef] [Google Scholar]
  10. Knoblauch, C., Sahm, K. & Jørgensen, B. B.(1999). Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigus oceanense gen. nov., sp. nov., Desulfofrigus fragile sp. nov., Desulfofaba gelida gen. nov., sp. nov., Desulfotalea psychrophila gen. nov., sp. nov. and Desulfotalea arctica sp. nov. Int J Syst Bacteriol 49, 1631–1643.[CrossRef] [Google Scholar]
  11. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  12. Kuever, J., Rainey, F. A. & Widdel, F.(2005). Class IV. Deltaproteobacteria class nov. In Bergey's Manual of Systematic Bacteriology, vol. 2, part C, 2nd edn, pp. 922–1144. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Miller, L. T.(1982). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  14. Miyagawa, E., Azuma, R. & Suto, E.(1979). Cellular fatty acid composition in Gram-negative obligately anaerobic rods. J Gen Appl Microbiol 25, 41–51.[CrossRef] [Google Scholar]
  15. Moore, L. V. H., Bourne, D. M. & Moore, W. E. C.(1994). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol 44, 338–347.[CrossRef] [Google Scholar]
  16. Nakamoto, M., Ueki, A. & Ueki, K.(1996). Physiological properties of a sulfate-reducing bacterium isolated from municipal sewage sludge and its possible role as a syntrophic acidogen in the ecosystem. J Gen Appl Microbiol 42, 109–120.[CrossRef] [Google Scholar]
  17. Rabus, R., Hansen, T. & Widdel, F.(2000). Dissimilatory sulfate- and sulfur-reducing prokaryotes. In The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3rd edn. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Sørensen, J., Christensen, D. & Jørgensen, B. B.(1981). Volatile fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl Environ Microbiol 42, 5–11. [Google Scholar]
  20. Suzuki, D., Ueki, A., Amaishi, A. & Ueki, K.(2007a).Desulfopila aestuarii gen. nov., sp. nov., a novel, Gram-negative, rod-like, sulfate-reducing bacterium isolated from an estuarine sediment in Japan. Int J Syst Evol Microbiol 57, 520–526.[CrossRef] [Google Scholar]
  21. Suzuki, D., Ueki, A., Amaishi, A. & Ueki, K.(2007b).Desulfobulbus japonicus sp. nov., a novel, Gram-negative, propionate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan. Int J Syst Evol Microbiol 57, 849–855.[CrossRef] [Google Scholar]
  22. Suzuki, D., Ueki, A., Amaishi, A. & Ueki, K.(2007c). Diversity of substrate utilization and growth characteristics of sulfate-reducing bacteria isolated from estuarine sediment in Japan. J Gen Appl Microbiol 53, 119–132.[CrossRef] [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  24. Ueki, A. & Suto, T.(1979). Cellular fatty acid composition of sulfate-reducing bacteria. J Gen Appl Microbiol 25, 185–196.[CrossRef] [Google Scholar]
  25. Ueki, A., Minato, H., Azuma, R. & Suto, T.(1980). Enumeration and isolation of anaerobic bacteria in sewage digester fluids: enumeration of sulfate-reducers by the anaerobic roll tube method. J Gen Appl Microbiol 26, 25–35.[CrossRef] [Google Scholar]
  26. Ueki, A., Matsuda, K. & Ohtsuki, C.(1986). Sulfate reduction in the anaerobic digestion of animal waste. J Gen Appl Microbiol 32, 111–123.[CrossRef] [Google Scholar]
  27. Ueki, A., Akasaka, H., Suzuki, D., Hattori, S. & Ueki, K.(2006a).Xylanibacter oryzae gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, xylanolytic bacterium isolated from rice-plant residue in flooded rice-field soil in Japan. Int J Syst Evol Microbiol 56, 2215–2221.[CrossRef] [Google Scholar]
  28. Ueki, A., Akasaka, H., Suzuki, D. & Ueki, K.(2006b).Paludibacter propionicigenes gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, propionate-producing bacterium isolated from rice-plant residue in irrigated rice-field soil in Japan. Int J Syst Evol Microbiol 56, 39–44.[CrossRef] [Google Scholar]
  29. Widdel, F. & Bak, F.(1992). Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes, pp. 3352–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  30. Widdel, F., Kohring, G.-W. & Mayer, F.(1983). Studies of dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov. and sp. nov. and Desulfonema magnum sp. nov. Arch Microbiol 134, 286–294.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65306-0
Loading
Desulfoluna butyratoxydans gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan
Int J Syst Evol Microbiol 58, 826 (2008); https://doi.org/10.1099/ijs.0.65306-0
/content/journal/ijsem/10.1099/ijs.0.65306-0
/content/journal/ijsem/10.1099/ijs.0.65306-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error