RT Journal Article SR Electronic(1) A1 Tóth, Erika M. A1 Kéki, Zsuzsa A1 Homonnay, Zalán G. A1 Borsodi, Andrea K. A1 Márialigeti, Károly A1 Schumann, PeterYR 2008 T1 Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera) JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 1 SP 78 OP 83 DO https://doi.org/10.1099/ijs.0.65305-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, rod-shaped or coccoid, yellow-pigmented bacterial strain, D287T, was isolated from the water flea Daphnia cucullata (Crustacea: Cladocera) collected from Lake Balaton in Hungary. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons revealed that the strain represented a distinct lineage within the cluster of the genera Nocardioides and Marmoricola. The following characteristics were consistent with the affiliation of strain D287T to the genus Nocardioides: peptidoglycan based on ll-2,6-diaminopimelic acid, MK-8(H4) as the major menaquinone, iso-C16 : 0 as the predominant cellular fatty acid, the presence of phosphatidylglycerol and diphosphatidylglycerol and a DNA G+C content of 69.9 mol%. Owing to characteristic differences in physiological traits and levels of 16S rRNA gene sequence similarity to its phylogenetically closest neighbours that were below 97 %, strain D287T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daphniae sp. nov. is proposed. The type strain is D287T (=DSM 18664T=CCM 7403T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65305-0