1887

Abstract

Four Gram-positive, non-motile, non-spore-forming bacterial strains, LC4, LC6, LC10 and LC13, were isolated from a filtration substrate made from trass, a volcanic rock, and their taxonomic positions were investigated by a polyphasic taxonomic approach. The novel strains grew over a temperature range of 5–40 °C, at pH values of 6–11 and in the presence of 3–7 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed the novel strains formed a distinct evolutionary lineage within the genus . Chemotaxonomic analyses demonstrated that the major menaquinone was MK-9(H), a menaquinone typical of the group. The major fatty acid was anteiso-C and the major amino acid present in the cell-wall peptidoglycan was -lysine. These observations supported the affiliation of the novel strains to the genus . On the basis of their morphological, physiological and genotypic characteristics, the new isolates are considered to represent four novel species of the genus , for which the names sp. nov. (type strain LC4=IAM 15382=CCTCC AB 206012), sp. nov. (type strain LC6=IAM 15383=CCTCC AB 206013), sp. nov. (type strain LC10=IAM 15385=CCTCC AB 206017) and sp. nov. (type strain LC13=IAM 15386=CCTCC AB 206018) are proposed.

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2009-04-01
2021-07-23
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