1887

Abstract

Three novel strains, designated Gsoil 034, Gsoil 060 and Jip13, isolated from soil from a ginseng field and compost in South Korea, were characterized using a polyphasic approach to clarify their taxonomic positions. These isolates were found to be Gram-negative, aerobic and heterotrophic, non-spore-forming, non-motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these three isolates formed a cluster with the monospecific genus within the family but were clearly separated from . The 16S rRNA gene sequence similarities between these isolates and the type strain of were in the range 88.5–90.1 %. Phenotypic and chemotaxonomic data (MK-7 as the major isoprenoid quinone and iso-C 2-OH and/or C 7, iso-C and iso-C 3-OH as the major fatty acids) supported the affiliation of these strains to the genus . However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from species with validly published names. Therefore strains Gsoil 034, Gsoil 060 and Jip13 represent three novel species of the genus , for which the names sp. nov. (type strain Gsoil 034 =KCTC 12645 =LMG 23492), sp. nov. (type strain Gsoil 060 =KCTC 12646 =LMG 23491) and sp. nov. (type strain Jip13 =KCTC 12644 =LMG 23494) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65299-0
2008-05-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/5/1123.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65299-0&mimeType=html&fmt=ahah

References

  1. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  4. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  5. Hiraishi, A., Ueda, Y., Ishihara, J. & Mori, T. ( 1996; ). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42, 457–469.[CrossRef]
    [Google Scholar]
  6. Im, W.-T., Kang, M.-S., Park, H.-Y., Kim, M.-K. & Lee, S.-T. ( 2003; ). Culturable bacterial strain's diversity of environmental samples. In Proceedings of the International Meeting of the Federation of Korean Microbiological Societies, abstract B4023, p. 165. Seoul: Federation of Korean Microbiological Societies.
  7. Im, W.-T., Jung, H.-M., Cui, Y.-S., Liu, Q.-M., Zhang, S.-L. & Lee, S.-T. ( 2005; ). Cultivation of the three hundreds of bacterial species from soil of a ginseng field and mining the novel lineage bacteria. In Proceedings of the International Meeting of the Federation of Korean Microbiological Societies, abstract A035, p. 169. Seoul: Federation of Korean Microbiological Societies.
  8. Kim, M. K., Im, W.-T., Ohta, H., Lee, M. & Lee, S.-T. ( 2005; ). Sphingopyxis granuli sp. nov., a β-glucosidase producing bacterium in the family Sphingomonadaceae in α-4 subclass of the Proteobacteria. J Microbiol 43, 152–157.
    [Google Scholar]
  9. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Moore, D. D. & Dowhan, D. ( 1995; ). Preparation and analysis of DNA. In Current Protocols in Molecular Biology, pp. 2–11. Edited by F. W. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  13. Ntougias, S., Fasseas, C. & Zervakis, G. I. ( 2007; ). Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete. Int J Syst Evol Microbiol 57, 398–404.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65299-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65299-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error