RT Journal Article SR Electronic(1) A1 Montes, Ma Jesús A1 Bozal, Núria A1 Mercadé, ElenaYR 2008 T1 Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 6 SP 1346 OP 1349 DO https://doi.org/10.1099/ijs.0.65298-0 PB Microbiology Society, SN 1466-5034, AB Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3BT and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase- and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na+ and grew at NaCl concentrations of 1–15 % and temperatures between 4 and 42 °C. 16S rRNA gene sequence analysis placed strains M3BT and M3T within the genus Marinobacter. DNA–DNA hybridization experiments between the Antarctic isolate M3BT and type strains of phylogenetically related species, namely Marinobacter lipolyticus, Marinobacter flavimaris, Marinobacter sediminum, Marinobacter algicola, Marinobacter maritimus and Marinobacter koreensis, revealed levels of relatedness lower than 32 %. Strain M3T showed 99 % DNA relatedness to strain M3BT. The DNA G+C contents of M3BT and M3T were 57.1 and 57.4 mol%, respectively, and their major isoprenoid quinone was ubiquinone-9. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M3BT and M3T from strains of related Marinobacter species. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M3BT and M3T belong to the same genospecies and represent a novel species of the genus Marinobacter, for which the name Marinobacter guineae sp. nov. is proposed. The type strain is M3BT (=LMG 24048T=CECT 7243T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65298-0